ArticlePlant physiology2014
Dynamics of Male and Female Chromatin during Karyogamy in Rice Zygotes.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed, 67 citations in OpenAlex.
- Monitoring the fate of paternal mitochondria and their elimination in rice zygotes.Plant physiology · 2026Article
- Autonomous development and regeneration of isolated rice egg cells in vitro in a fertilization-independent manner.Journal of experimental botany · 2026Article
- Article
- Role of paternal Oryza sativa Baby Booms (OsBBMs) in initiating de novo gene expression and regulating early zygotic development in rice.The Plant journal : for cell and molecular biology · 2025Article
- Transcriptional dynamics during karyogamy in rice zygotes.Development (Cambridge, England) · 2025Article
- From gametes to zygote: Mechanistic advances and emerging possibilities in plant reproduction.Plant physiology · 2024Review
- The Feeder Effects of Cultured Rice Cells on the Early Development of Rice Zygotes.International journal of molecular sciences · 2023Article
- Nuclear Fusion in Yeast and Plant Reproduction.Plants (Basel, Switzerland) · 2023Article
- F-actin regulates the polarized secretion of pollen tube attractants in Arabidopsis synergid cells.The Plant cell · 2023Article
- Isolation of gametes and zygotes from Setaria viridis.Journal of plant research · 2022Article
- Dynamics of mitochondrial distribution during development and asymmetric division of rice zygotes.Plant reproduction · 2022Article
- Phylogenetically DistantFrontiers in plant science · 2022Article
- In Vitro Fertilization System Using Wheat Gametes by Electric Fusion.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Options for Engineering Apomixis in Plants.Frontiers in plant science · 2022Review
- Development and regeneration of wheat-rice hybrid zygotes produced by in vitro fertilization system.The New phytologist · 2021Article
- Insights into the molecular evolution of fertilization mechanism in land plants.Plant reproduction · 2021Review
- Regulatory functions of ROS dynamics via glutathione metabolism and glutathione peroxidase activity in developing rice zygote.The Plant journal : for cell and molecular biology · 2021Article
- Formins control dynamics of F-actin in the central cell ofPlant signaling & behavior · 2021Article
- Effect of Paternal Genome Excess on the Developmental and Gene Expression Profiles of Polyspermic Zygotes in Rice.Plants (Basel, Switzerland) · 2021Article
- Developmental and genomic architecture of plant embryogenesis: from model plant to crops.Plant communications · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In angiosperms, the conversion of an egg cell into a zygote involves two sequential gametic processes: plasmogamy, the fusion of the plasma membranes of male and female gametes, and karyogamy, the fusion of the gametic nuclei. In this study, the nuclei and nuclear membranes of rice (Oryza sativa) gametes were fluorescently labeled using histones 2B-green fluorescent protein/red fluorescent protein and Sad1/UNC-84-domain protein2-green fluorescent protein, respectively, which were heterologously expressed. These gametes were fused in vitro to produce zygotes, and the nuclei and nuclear membranes in the zygotes were observed during karyogamy. The results indicated that the sperm nucleus migrates adjacent to the egg nucleus 5 to 10 min after plasmogamy via an actin cytoskelton, and the egg chromatin then appears to move unidirectionally into the sperm nucleus through a possible nuclear connection. The enlargement of the sperm nucleus accompanies this possible chromatin remodeling. Then, 30 to 70 min after fusion, the sperm chromatin begins to decondense with the completion of karyogamy. Based on these observations, the development of early rice zygotes from plasmogamy to karyogamy was divided into eight stages, and using reverse transcription PCR analyses, paternal and de novo synthesized transcripts were separately detected in zygotes at early and late karyogamy stages, respectively.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.