ArticlePlant physiology2014
MYB5 and MYB14 Play Pivotal Roles in Seed Coat Polymer Biosynthesis in Medicago truncatula.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
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Who cites it
84 citing papers in PubMed, 150 citations in OpenAlex.
- Evolutionary and functional divergence of MYB regulatory networks in flavonoid biosynthesis across Poaceae, non-Poaceae monocots, and eudicot horticultural plants.Horticulture research · 2026Review
- Proanthocyanidins: structure, biosynthesis, regulation, and structure-activity relationships.aBIOTECH · 2026Review
- Molecular Insights intoPlants (Basel, Switzerland) · 2026Article
- DcMYB5 transcription factor regulates the biosynthesis of diosgenin by directly repressing CYP90B gene expression in Dioscorea composita.Plant cell reports · 2026Article
- Integrated multi-omics elucidates StAN1 as a key regulator reprogramming flavonoid partitioning and co-regulatory networks in potato tubers.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Evolutionary dynamics of the proanthocyanidin biosynthesis gene LAR.BMC genomics · 2025Article
- Evolution of short trichomes and long hairs on Nigella petals through co-option of bHLH and non-MIXTA MYB genes.Nature communications · 2025Article
- The TaMYB44-TaMYB1 module regulates grain amylose biosynthesis and flour viscosity in wheat.Plant communications · 2025Article
- Mutations of two AhTT8 genes reduce the accumulation of anthocyanins and confer the white testa of peanut (Arachis hypogaea L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Biosynthesis and Regulatory Mechanisms of Plant Flavonoids: A Review.Plants (Basel, Switzerland) · 2025Review
- Ethylene modulates the phenylpropanoid pathway by enhancingHorticulture research · 2025Article
- Molecular insights into TT2-type MYB regulators illuminate the complexity of floral flavonoids biosynthesis inHorticulture research · 2025Article
- Infiltration-RNAseq Reveals Enhanced Defense Responses inPlants (Basel, Switzerland) · 2025Article
- Metabolomics and Transcriptomics Jointly Explore the Mechanism of Pod Color Variation in Purple Pod Pea.Current issues in molecular biology · 2025Article
- Molecular insights into postharvest seed coat darkening in common beans: a look beyond theFrontiers in plant science · 2025Article
- The Lotus corniculatus MYB5 functions as a master regulator in proanthocyanidin biosynthesis and bioengineering.Plant cell reports · 2024Article
- Distinctive acidity in citrus fruit is linked to loss of proanthocyanidin biosynthesis.iScience · 2024Article
- T and Z, partial seed coat patterning genes in common bean, provide insight into the structure and protein interactions of a plant MBW complex.G3 (Bethesda, Md.) · 2024Article
- Molecular evolution analysis of MYB5 in Brassicaceae with specific focus on seed coat color of Brassica napus.BMC plant biology · 2024Article
- Multiple bHLH/MYB-based protein complexes regulate proanthocyanidin biosynthesis in the herbage of Lotus spp.Planta · 2023Article
24 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Arabidopsis (Arabidopsis thaliana), the major MYB protein regulating proanthocyanidin (PA) biosynthesis is TT2, named for the transparent testa phenotype of tt2 mutant seeds that lack PAs in their coats. In contrast, the MYB5 transcription factor mainly regulates seed mucilage biosynthesis and trichome branching, with only a minor role in PA biosynthesis. We here characterize MYB5 and MYB14 (a TT2 homolog) in the model legume Medicago truncatula. Overexpression of MtMYB5 or MtMYB14 strongly induces PA accumulation in M. truncatula hairy roots, and both myb5 and myb14 mutants of M. truncatula exhibit darker seed coat color than wild-type plants, with myb5 also showing deficiency in mucilage biosynthesis. myb5 mutant seeds have a much stronger seed color phenotype than myb14. The myb5 and myb14 mutants accumulate, respectively, about 30% and 50% of the PA content of wild-type plants, and PA levels are reduced further in myb5 myb14 double mutants. Transcriptome analyses of overexpressing hairy roots and knockout mutants of MtMYB5 and MtMYB14 indicate that MtMYB5 regulates a broader set of genes than MtMYB14. Moreover, we demonstrate that MtMYB5 and MtMYB14 physically interact and synergistically activate the promoters of anthocyanidin reductase and leucoanthocyanidin reductase, the key structural genes leading to PA biosynthesis, in the presence of MtTT8 and MtWD40-1. Our results provide new insights into the complex regulation of PA and mucilage biosynthesis in M. truncatula.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.