ArticleG3 (Bethesda, Md.)2014
Proteomic analysis reveals CACN-1 is a component of the spliceosome in Caenorhabditis elegans.
Article in G3 (Bethesda, Md.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
11 citing papers in PubMed, 13 citations in OpenAlex.
- Article
- Mechanisms of germ cell survival and plasticity in Caenorhabditis elegans.Biochemical Society transactions · 2022Review
- Border cell polarity and collective migration require the spliceosome component Cactin.The Journal of cell biology · 2022Article
- The Interplay of Cohesin and RNA Processing Factors: The Impact of Their Alterations on Genome Stability.International journal of molecular sciences · 2022Review
- mRNA Editing, Processing and Quality Control inGenetics · 2020Review
- Tandem Affinity Purification and Mass Spectrometry (TAP-MS) for the Analysis of Protein Complexes.Current protocols in protein science · 2019Article
- Sde2 is an intron-specific pre-mRNA splicing regulator activated by ubiquitin-like processing.The EMBO journal · 2018Article
- Invading, Leading and Navigating Cells inGenetics · 2018Review
- Regulating distal tip cell migration in space and time.Mechanisms of development · 2017Review
- RNAi Screen Identifies Novel Regulators of RNP Granules in the Caenorhabditis elegans Germ Line.G3 (Bethesda, Md.) · 2016Article
- CACN-1 is required in the Caenorhabditis elegans somatic gonad for proper oocyte development.Developmental biology · 2016Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Cell migration is essential for embryonic development and tissue formation in all animals. cacn-1 is a conserved gene of unknown molecular function identified in a genome-wide screen for genes that regulate distal tip cell migration in the nematode worm Caenorhabditis elegans. In this study we take a proteomics approach to understand CACN-1 function. To isolate CACN-1-interacting proteins, we used an in vivo tandem-affinity purification strategy. Tandem-affinity purification-tagged CACN-1 complexes were isolated from C. elegans lysate, analyzed by mass spectrometry, and characterized bioinformatically. Results suggest significant interaction of CACN-1 with the C. elegans spliceosome. All of the identified interactors were screened for distal tip cell migration phenotypes using RNAi. Depletion of many of these factors led to distal tip cell migration defects, particularly a failure to stop migrating, a phenotype commonly seen in cacn-1 deficient animals. The results of this screen identify eight novel regulators of cell migration and suggest CACN-1 may participate in a protein network dedicated to high-fidelity gonad development. The composition of proteins comprising the CACN-1 network suggests that this critical developmental module may exert its influence through alternative splicing or other post-transcriptional gene regulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.