ArticleG3 (Bethesda, Md.)2014
Histone H2B ubiquitination promotes the function of the anaphase-promoting complex/cyclosome in Schizosaccharomyces pombe.
Article in G3 (Bethesda, Md.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 16 citations in OpenAlex.
- Distinct roles of histone H2B ubiquitination at promoters and coding regions of Pol II-transcribed stress genes.Genome biology · 2025Article
- Nucleosomes represent a crucial target for the intra-S phase checkpoint in response to replication stress.Science advances · 2025Article
- Quantitative Assessment of Histone H2B Monoubiquitination in Yeast Using Immunoblotting.Methods and protocols · 2022Article
- Localization of the ubiquitin ligase Dma1 to the fission yeast contractile ring is modulated by phosphorylation.FEBS letters · 2021Article
- Cdk1 phosphorylation of fission yeast paxillin inhibits its cytokinetic ring localization.Molecular biology of the cell · 2021Article
- Fission yeast Opy1 is an endogenous PI(4,5)PJournal of cell science · 2020Article
- A balance of deubiquitinating enzymes controls cell cycle entry.Molecular biology of the cell · 2018Article
- Relief of the Dma1-mediated checkpoint requires Dma1 autoubiquitination and dynamic localization.Molecular biology of the cell · 2018Article
- Phosphoinositide-mediated ring anchoring resists perpendicular forces to promote medial cytokinesis.The Journal of cell biology · 2017Article
- A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.Molecular & cellular proteomics : MCP · 2015Article
- Role of Ccr4-Not complex in heterochromatin formation at meiotic genes and subtelomeres in fission yeast.Epigenetics & chromatin · 2015Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Ubiquitination and deubiquitination of proteins are reciprocal events involved in many cellular processes, including the cell cycle. During mitosis, the metaphase to anaphase transition is regulated by the ubiquitin ligase activity of the anaphase-promoting complex/cyclosome (APC/C). Although the E3 ubiquitin ligase function of the APC/C has been well characterized, it is not clear whether deubiquitinating enzymes (DUBs) play a role in reversing APC/C substrate ubiquitination. Here we performed a genetic screen to determine what DUB, if any, antagonizes the function of the APC/C in the fission yeast Schizosaccharomyces pombe. We found that deletion of ubp8, encoding the Spt-Ada-Gcn5-Acetyl transferase (SAGA) complex associated DUB, suppressed temperature-sensitive phenotypes of APC/C mutants cut9-665, lid1-6, cut4-533, and slp1-362. Our analysis revealed that Ubp8 antagonizes APC/C function in a mechanism independent of the spindle assembly checkpoint and proteasome activity. Notably, suppression of APC/C mutants was linked to loss of Ubp8 catalytic activity and required histone H2B ubiquitination. On the basis of these data, we conclude that Ubp8 antagonizes APC/C function indirectly by modulating H2B ubiquitination status.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.