ArticleMicrobial cell factories2014
Production of medium chain length polyhydroxyalkanoate in metabolic flux optimized Pseudomonas putida.
Article in Microbial cell factories, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 125 citations in OpenAlex.
- Biosynthesis of medium-chain length polyhydroxyalkanoates from pre-treated food wastewater.Biotechnology for biofuels and bioproducts · 2026Article
- Bacterial species-structure-property relationships of polyhydroxyalkanoate biopolymers produced on simple sugars for thin film applications.Microbial cell factories · 2025Article
- Modification of Glucose Metabolic Pathway to Enhance Polyhydroxyalkanoate Synthesis inCurrent issues in molecular biology · 2024Article
- Polyhydroxyalkanoates: Medical Applications and Potential for Use in Dentistry.Materials (Basel, Switzerland) · 2024Review
- Advances in Microbial Biotechnology for Sustainable Alternatives to Petroleum-Based Plastics: A Comprehensive Review of Polyhydroxyalkanoate Production.Microorganisms · 2024Review
- Medium Chain Length Polyhydroxyalkanoate Production by Engineered Pseudomonas gessardii Using Acetate-formate as Carbon Sources.Journal of microbiology (Seoul, Korea) · 2024Article
- Recent Challenges and Trends of Polyhydroxyalkanoate Production by Extremophilic Bacteria Using Renewable Feedstocks.Polymers · 2023Review
- Rational engineering of natural polyhydroxyalkanoates producing microorganisms for improved synthesis and recovery.Microbial biotechnology · 2023Review
- Streamlining of a synthetic co-culture towards an individually controllable one-pot process for polyhydroxyalkanoate production from light and COEngineering in life sciences · 2023Article
- Microbial Valorization of Lignin to Bioplastic by Genome-ReducedFrontiers in microbiology · 2022Article
- Reconstruction and optimization of aFrontiers in bioengineering and biotechnology · 2022Article
- Channelling carbon flux through the meta-cleavage route for improved poly(3-hydroxyalkanoate) production from benzoate and lignin-based aromatics in Pseudomonas putida H.Microbial biotechnology · 2021Article
- Post-Transcriptional Control in the Regulation of Polyhydroxyalkanoates Synthesis.Life (Basel, Switzerland) · 2021Review
- Sea-Ice BacteriaApplied and environmental microbiology · 2021Article
- Micro-aerobic production of isobutanol with engineeredEngineering in life sciences · 2021Article
- Adaptive Laboratory Evolution Restores Solvent Tolerance in Plasmid-Cured Pseudomonas putida S12: a Molecular Analysis.Applied and environmental microbiology · 2021Article
- Enhancement of polyhydroxyalkanoate production by co-feeding lignin derivatives with glycerol in Pseudomonas putida KT2440.Biotechnology for biofuels · 2021Article
- Recent advances in constructing artificial microbial consortia for the production of medium-chain-length polyhydroxyalkanoates.World journal of microbiology & biotechnology · 2021Review
- Optimization of a Two-Species Microbial Consortium for Improved Mcl-PHA Production From Glucose-Xylose Mixtures.Frontiers in bioengineering and biotechnology · 2021Article
- Fed-BatchFrontiers in bioengineering and biotechnology · 2021Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPseudomnas putida is a natural producer of medium chain length polyhydroxyalkanoates (mcl-PHA), a polymeric precursor of bioplastics. A two-fold increase of mcl-PHA production via inactivation of the glucose dehydrogenase gene gcd, limiting the metabolic flux towards side products like gluconate was achieved before. Here, we investigated the overproduction of enzymes catalyzing limiting steps of mcl-PHA precursor formation.
resultsA genome-based in silico model for P. putida KT2440 metabolism was employed to identify potential genetic targets to be engineered for the improvement of mcl-PHA production using glucose as sole carbon source. Here, overproduction of pyruvate dehydrogenase subunit AcoA in the P. putida KT2440 wild type and the Δgcd mutant strains led to an increase of PHA production. In controlled bioreactor batch fermentations PHA production was increased by 33% in the acoA overexpressing wild type and 121% in the acoA overexpressing Δgcd strain in comparison to P. putida KT2440. Overexpression of pgl-encoding 6-phosphoglucolactonase did not influence PHA production. Transcriptome analyses of engineered PHA producing P. putida in comparison to its parental strains revealed the induction of genes encoding glucose 6-phosphate dehydrogenase and pyruvate dehydrogenase. In addition, NADPH seems to be quantitatively consumed for efficient PHA synthesis, since a direct relationship between low levels of NADPH and high concentrations of the biopolymer were observed. In contrast, intracellular levels of NADH were found increased in PHA producing organisms.
conclusionProduction of mcl-PHAs was enhanced in P. putida when grown on glucose via overproduction of a pyruvate dehydrogenase subunit (AcoA) in combination with a deletion of the glucose dehydrogenase (gcd) gene as predicted by in silico elementary flux mode analysis.
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