ArticleScientific reports2014
Tyrosinase-catalyzed site-specific immobilization of engineered C-phycocyanin to surface.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 29 citations in OpenAlex.
- Harnessing Frémy's salt for tyrosine-directed bioconjugations.RSC advances · 2026Article
- Enzymes in "Green" Synthetic Chemistry: Laccase and Lipase.Molecules (Basel, Switzerland) · 2024Review
- Designed protein multimerization and polymerization for functionalization of proteins.Biotechnology letters · 2022Review
- Application of bacterial tyrosinases in organic synthesis.World journal of microbiology & biotechnology · 2021Review
- Reconstruction of the absorption spectrum of Synechocystis sp. PCC 6803 optical mutants from the in vivo signature of individual pigments.Photosynthesis research · 2021Article
- Complete inclusion of bioactive molecules and particles in polydimethylsiloxane: a straightforward process under mild conditions.Scientific reports · 2019Article
- From Protein Features to Sensing Surfaces.Sensors (Basel, Switzerland) · 2018Review
- ortho-Quinones and Analogues Thereof: Highly Reactive Intermediates for Fast and Selective Biofunctionalization.Chemistry (Weinheim an der Bergstrasse, Germany) · 2018Review
- Inducible, Site-Specific Protein Labeling by Tyrosine Oxidation-Strain-Promoted (4 + 2) Cycloaddition.Bioconjugate chemistry · 2017Article
- Protein retention on plasma-treated hierarchical nanoscale gold-silver platform.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enzymatic crosslinking of proteins is often limited by the steric availability of the target residues, as of tyrosyl side chains in the case of tyrosinase. Carrying an N-terminal peptide-tag containing two tyrosine residues, the fluorescent protein C-phycocyanin HisCPC from Synechocystis sp. PCC6803 was crosslinked to fluorescent high-molecular weight forms with tyrosinase. Crosslinking with tyrosinase in the presence of L-tyrosine produced non fluorescent high-molecular weight products. Incubated in the presence of tyrosinase, HisCPC could also be immobilized to amino-modified polystyrene beads thus conferring a blue fluorescence. Crosslinking and immobilization were site-specific as both processes required the presence of the N-terminal peptide in HisCPC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.