ArticleJournal of industrial microbiology & biotechnology2014
A novel approach for improving the yield of Bacillus subtilis transglutaminase in heterologous strains.
Article in Journal of industrial microbiology & biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 39 citations in OpenAlex.
- Enhancing substrate specificity of microbial transglutaminase for precise nanobody labeling.Synthetic and systems biotechnology · 2025Article
- Optimization of a novel expression system for recombinant protein production in CHO cells.Scientific reports · 2024Article
- Synthesis and Properties of Injectable Hydrogel for Tissue Filling.Pharmaceutics · 2024Review
- Recombinant Streptomyces netropsis transglutaminase expressed in Komagataella phaffii (Pichia pastoris) and applied in plant-based chicken nugget.World journal of microbiology & biotechnology · 2023Article
- Crosslinking Mechanism on a NovelFoods (Basel, Switzerland) · 2022Article
- Active secretion of a thermostable transglutaminase variant in Escherichia coli.Microbial cell factories · 2022Article
- Production of transglutaminase in glutathione-producing recombinant Saccharomyces cerevisiae.AMB Express · 2021Article
- A recent update on the use of microbial transglutaminase for the generation of biotherapeutics.World journal of microbiology & biotechnology · 2020Review
- Transglutaminases: part I-origins, sources, and biotechnological characteristics.World journal of microbiology & biotechnology · 2020Review
- Design of a glutamine substrate tag enabling protein labelling mediated by Bacillus subtilis transglutaminase.PloS one · 2018Article
- Transglutaminases in Dysbiosis As Potential Environmental Drivers of Autoimmunity.Frontiers in microbiology · 2017Article
- Draft Genome Sequence of Bacillus tequilensis Strain FJAT-14262a.Genome announcements · 2015Article
- Role of fatty acids in Bacillus environmental adaptation.Frontiers in microbiology · 2015Review
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Authors and funding
6 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The transglutaminase (BTG) from Bacillus subtilis is considered to be a new type of transglutaminase for the food industry. Given that the BTG gene only encodes a mature peptide, the expression of BTG in heterologous microbial hosts could affect their normal growth due to BTG's typical transglutaminase activity which can catalyze cross-linking of proteins in the cells. Therefore, we developed a novel approach to suppress BTG activity and reduce the toxicity on microbial hosts, thus improving BTG yield. Genes encoding the respective regions of transglutaminase propeptide from seven species of Streptomyces were fused to the N-terminal of the BTG gene to produce fusion proteins. We found that all the fused propeptides could suppress BTG activity. Importantly, BTG activity could be completely restored after the removal of the propeptides by proteolytic cleavage. Of the seven propeptides tested, the propeptide proD from Streptomyces caniferus had the strongest suppressive effect on BTG activity (70 % of the activity suppressed). Moreover, fusion protein proD-BTG (containing proD) also exhibited the highest yield which was more than twofold of the expression level of BTG in an active form in Escherichia coli. Secretion expression of BTG and proD-BTG in Corynebacterium glutamicum further showed that our novel approach was suitable for the efficient BTG expression, thus providing a valuable platform for further optimization of large-scale BTG production.
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