Evidence map›Paper›PMID 24947398›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2014

Probing the role of the actin cytoskeleton during regulated exocytosis by intravital microscopy.

Oleg Milberg, Muhibullah Tora, Akiko Shitara, Taishin Takuma, Andrius Masedunskas, Roberto Weigert

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Oleg MilbergIntracellular Membrane Trafficking Unit, Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, 30 Convent Dr. 303A, 20892-4340, Bethesda, MD, USA.
Muhibullah Tora
Akiko Shitara
Taishin Takuma
Andrius Masedunskas
Roberto Weigert

Funding

Molecular Mechanisms regulating membrane trafficking in salivary glandsZIADE000717 · NIDCR · NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH · PI WEIGERT, ROBERTO · 2009 to 2015
$9.2M
Intramural NIH HHS ZIA DE000717
6 · The paper itself

Abstract

The actin cytoskeleton plays a fundamental role in controlling several steps during regulated exocytosis. Here, we describe a combination of procedures that are aimed at studying the dynamics and the mechanism of the actin cytoskeleton in the salivary glands of live rodents, a model for exocrine secretion. Our approach relies on intravital microscopy, an imaging technique that enables imaging biological events in live animals at a subcellular resolution, and it is complemented by the use of pharmacological agents and indirect immunofluorescence in the salivary tissue.

Indexed as

ActinsAnimalsExocytosisFemaleMaleMiceMicroscopyMicroscopy, FluorescenceSalivary GlandsSecretory VesiclesActins

Identifiers

PMID24947398
PMCPMC4699287

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.