Evidence map›Paper›PMID 24946775›Full record

ArticleBMC microbiology2014

Population typing of the causal agent of cassava bacterial blight in the Eastern Plains of Colombia using two types of molecular markers.

César A Trujillo, Nathalia Arias-Rojas, Lucie Poulin, César A Medina, Anibal Tapiero, Silvia Restrepo, Ralf Koebnik, Adriana J Bernal

Abstract read
In one paragraph

Article in BMC microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

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8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Review
  3. Molecular Epidemiology ofMicroorganisms · 2021
    Article
  4. Article
  5. Genome-Scale Metabolic Model ofFrontiers in genetics · 2020
    Article
  6. Article
  7. Article
  8. Article
4 · The record

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5 · Who and what money

Authors and funding

8 authors.

César A Trujillo
Nathalia Arias-Rojas
Lucie Poulin
César A Medina
Anibal Tapiero
Silvia Restrepo
Ralf Koebnik
Adriana J BernalLaboratorio de Micología y Fitopatología Uniandes (LAMFU), Universidad de Los Andes, Bogotá, Colombia. abernal@uniandes.edu.co.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMolecular typing of pathogen populations is an important tool for the development of effective strategies for disease control. Diverse molecular markers have been used to characterize populations of Xanthomonas axonopodis pv. manihotis (Xam), the main bacterial pathogen of cassava. Recently, diversity and population dynamics of Xam in the Colombian Caribbean coast were estimated using AFLPs, where populations were found to be dynamic, diverse and with haplotypes unstable across time. Aiming to examine the current state of pathogen populations located in the Colombian Eastern Plains, we also used AFLP markers and we evaluated the usefulness of Variable Number Tandem Repeats (VNTRs) as new molecular markers for the study of Xam populations.

resultsThe population analyses showed that AFLP and VNTR provide a detailed and congruent description of Xam populations from the Colombian Eastern Plains. These two typing strategies clearly separated strains from the Colombian Eastern Plains into distinct populations probably because of geographical distance. Although the majority of analyses were congruent between typing markers, fewer VNTRs were needed to detect a higher number of genetic populations of the pathogen as well as a higher genetic flow among sampled locations than those detected by AFLPs.

conclusionsThis study shows the advantages of VNTRs over AFLPs in the surveillance of pathogen populations and suggests the implementation of VNTRs in studies that involve large numbers of Xam isolates in order to obtain a more detailed overview of the pathogen to improve the strategies for disease control.

Indexed as

Genetic VariationAmplified Fragment Length Polymorphism AnalysisCluster AnalysisColombiaDNA, BacterialGenotypeManihotMinisatellite RepeatsMolecular Sequence DataMolecular TypingPhylogenyPlant DiseasesSequence Analysis, DNAXanthomonas axonopodisDNA, Bacterial

Identifiers

PMID24946775
PMCPMC4071214

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.