ArticleMolecular microbiology2014
Comparative analysis of the secretion capability of early and late flagellar type III secretion substrates.
Article in Molecular microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- Tumour-specific delivery of ω-conotoxin MVIIA via engineered Salmonella for safe anticancer therapy.Journal of advanced research · 2026Article
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- Anti-cancer effect of interleukin-2 fused to flagellin expressed by tumor-targeting Salmonella.Oncogene · 2025Article
- FlhE functions as a chaperone to prevent formation of periplasmic flagella in Gram-negative bacteria.Nature communications · 2024Article
- Zinc effects on bacteria: insights frommSystems · 2023Article
- Plasmid-encoded lactose metabolism and mobilized colistin resistance (Microbial genomics · 2023Article
- Article
- Targeting early proximal-rod component substrate FlgB to FlhB for flagellar-type III secretion in Salmonella.PLoS genetics · 2022Article
- Structure and Assembly of the Bacterial Flagellum.Sub-cellular biochemistry · 2022Review
- Molecular Determinants of Filament Capping Proteins Required for the Formation of Functional Flagella in Gram-Negative Bacteria.Biomolecules · 2021Article
- Control of membrane barrier during bacterial type-III protein secretion.Nature communications · 2021Article
- Multiple Roles of Flagellar Export Chaperones for Efficient and Robust Flagellar Filament Formation inFrontiers in microbiology · 2021Article
- In Vitro Autonomous Construction of the Flagellar Axial Structure in Inverted Membrane Vesicles.Biomolecules · 2020Article
- Engineering the flagellar type III secretion system: improving capacity for secretion of recombinant protein.Microbial cell factories · 2019Article
- Developing Gram-negative bacteria for the secretion of heterologous proteins.Microbial cell factories · 2018Review
- Assembly Order of Flagellar Rod Subunits in Bacillus subtilis.Journal of bacteriology · 2018Article
- RNA Localization in Bacteria.Microbiology spectrum · 2018Review
- Flagella-mediated secretion of a novelCommunications biology · 2018Article
- Type III-Dependent Translocation of HrpB2 by a Nonpathogenic hpaABC Mutant of the Plant-Pathogenic Bacterium Xanthomonas campestris pv. vesicatoria.Applied and environmental microbiology · 2016Article
- The CsrA-FliW network controls polar localization of the dual-function flagellin mRNA in Campylobacter jejuni.Nature communications · 2016Article
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Authors and funding
3 authors at 2 institutions in 3 countries.
Funding
Abstract
A remarkable feature of the flagellar-specific type III secretion system (T3SS) is the selective recognition of a few substrate proteins among the many thousand cytoplasmic proteins. Secretion substrates are divided into two specificity classes: early substrates secreted for hook-basal body (HBB) construction and late substrates secreted after HBB completion. Secretion was reported to require a disordered N-terminal secretion signal, mRNA secretion signals within the 5'-untranslated region (5'-UTR) and for late substrates, piloting proteins known as the T3S chaperones. Here, we utilized translational β-lactamase fusions to probe the secretion efficacy of the N-terminal secretion signal of fourteen secreted flagellar substrates in Salmonella enterica. We observed a surprising variety in secretion capability between flagellar proteins of the same secretory class. The peptide secretion signals of the early-type substrates FlgD, FlgF, FlgE and the late-type substrate FlgL were analysed in detail. Analysing the role of the 5'-UTR in secretion of flgB and flgE revealed that the native 5'-UTR substantially enhanced protein translation and secretion. Based on our data, we propose a multicomponent signal that drives secretion via the flagellar T3SS. Both mRNA and peptide signals are recognized by the export apparatus and together with substrate-specific chaperones allowing for targeted secretion of flagellar substrates.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.