ArticlePLoS genetics2014
An operon of three transcriptional regulators controls horizontal gene transfer of the integrative and conjugative element ICEclc in Pseudomonas knackmussii B13.
Article in PLoS genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Horizontal transfer of ICEJournal of bacteriology · 2026Article
- Article
- Orchestrated long-distance gene activation by a ParB-like BisD-CTP DNA clamp in low-frequency transfer competence development in Pseudomonas putida.Nucleic acids research · 2025Article
- The Role of Genomic Islands in the Pathogenicity and Evolution of Plant-Pathogenic Gammaproteobacteria.Microorganisms · 2025Review
- Diversity and evolution of an abundant ICEmSphere · 2023Article
- Diversity and Evolution of Integrative and Conjugative Elements Involved in Bacterial Aromatic Compound Degradation and Their Utility in Environmental Remediation.Microorganisms · 2023Review
- A VirB4 ATPase of the mobile accessory genome orchestrates core genome-encoded features of physiology, metabolism, and virulence ofFrontiers in cellular and infection microbiology · 2023Article
- A bistable prokaryotic differentiation system underlying development of conjugative transfer competence.PLoS genetics · 2022Article
- An epigenetic switch activates bacterial quorum sensing and horizontal transfer of an integrative and conjugative element.Nucleic acids research · 2022Article
- Article
- A novel system of bacterial cell division arrest implicated in horizontal transmission of an integrative and conjugative element.PLoS genetics · 2019Article
- Article
- Physiological and transcriptome changes induced by Pseudomonas putida acquisition of an integrative and conjugative element.Scientific reports · 2018Article
- The Integrative Conjugative ElementFrontiers in microbiology · 2018Article
- The hidden life of integrative and conjugative elements.FEMS microbiology reviews · 2017Review
- Highly variable individual donor cell fates characterize robust horizontal gene transfer of an integrative and conjugative element.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Intraclonal genome diversity of the major Pseudomonas aeruginosa clones C and PA14.Environmental microbiology reports · 2016Article
- Correction: an operon of three transcriptional regulators controls horizontal gene transfer of the integrative and conjugative element ICEclc in Pseudomonas knackmussii B13.PLoS genetics · 2015Article
- The TetR-type MfsR protein of the integrative and conjugative element (ICE) ICEclc controls both a putative efflux system and initiation of ICE transfer.Journal of bacteriology · 2014Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The integrative and conjugative element ICEclc is a mobile genetic element in Pseudomonas knackmussii B13, and an experimental model for a widely distributed group of elements in Proteobacteria. ICEclc is transferred from specialized transfer competent cells, which arise at a frequency of 3-5% in a population at stationary phase. Very little is known about the different factors that control the transfer frequency of this ICE family. Here we report the discovery of a three-gene operon encoded by ICEclc, which exerts global control on transfer initiation. The operon consists of three consecutive regulatory genes, encoding a TetR-type repressor MfsR, a MarR-type regulator and a LysR-type activator TciR. We show that MfsR autoregulates expression of the operon, whereas TciR is a global activator of ICEclc gene expression, but no clear role was yet found for MarR. Deletion of mfsR increases expression of tciR and marR, causing the proportion of transfer competent cells to reach almost 100% and transfer frequencies to approach 1 per donor. mfsR deletion also caused a two orders of magnitude loss in population viability, individual cell growth arrest and loss of ICEclc. This indicates that autoregulation is an important feature maintaining ICE transfer but avoiding fitness loss. Bioinformatic analysis showed that the mfsR-marR-tciR operon is unique for ICEclc and a few highly related ICE, whereas tciR orthologues occur more widely in a large variety of suspected ICE among Proteobacteria.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.