ArticlePloS one2014
Analyzing receptor assemblies in the cell membrane using fluorescence anisotropy imaging with TIRF microscopy.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- Four in One: Parallel Determination of Optical Activity and Optical Anisotropy from Single Plasmonic Nanostructures.ACS nano · 2026Article
- Multidomain Coupling Governs FoxP1 Assembly and Nuclear Compartmentalization.bioRxiv : the preprint server for biology · 2026Article
- Bound or unbound: mapping and monitoring receptor oligomerization by time-resolved fluorescence live-cell imaging.Frontiers in molecular biosciences · 2026Article
- Total Internal Reflection Fluorescence (TIRF) Microscopy.Current protocols · 2022Review
- Increasing insulin measurement throughput by fluorescence anisotropy imaging immunoassays.Analytica chimica acta · 2022Article
- Kinetics of Histidine-Tagged Protein Association to Nickel-Decorated Liposome Surfaces.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- A concept for single-shot volumetric fluorescence imaging via orthogonally polarized excitation lattices.Scientific reports · 2019Article
- mmSTORM: Multimodal localization based super-resolution microscopy.Scientific reports · 2019Article
- Total internal reflection fluorescence anisotropy imaging microscopy: setup, calibration, and data processing for protein polymerization measurements in living cells.Methods and applications in fluorescence · 2017Article
- TestSTORM: Versatile simulator software for multimodal super-resolution localization fluorescence microscopy.Scientific reports · 2017Article
- Quantitative Affinity Determination by Fluorescence Anisotropy Measurements of Individual Nanoliter Droplets.Analytical chemistry · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Signaling within and between animal cells is controlled by the many receptor proteins in their membrane. They variously operate as trans-membrane monomers and homo- or hetero-dimers, and may assemble with ion-channels: analyses thereof are needed in studies of receptor actions in tissue physiology and pathology. Interactions between membrane proteins are detectable when pre-labeled with fluorophores, but a much fuller analysis is achievable via advanced optical techniques on living cells. In this context, the measurement of polarization anisotropy in the emitted fluorescence has been the least exploited. Here we demonstrate its methodology and particular advantages in the study of receptor protein assembly. Through excitation in both TIRF and EPI fluorescence illumination modes we are able to quantify and suppress contributions to the signal from extraneous intra-cellular fluorescence, and we show that the loss of fluorescence-polarization measured in membrane proteins reports on receptor protein assembly in real time. Receptor monomers and homo-dimers in the cell membrane can be analyzed quantitatively and for homo-dimers only a single fluorescent marker is needed, thus suppressing ambiguities that arise in alternative assays, which require multiple label moieties and which are thus subject to stoichiometric uncertainty.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.