ArticlePLoS genetics2014
Transcriptional control of steroid biosynthesis genes in the Drosophila prothoracic gland by ventral veins lacking and knirps.
Article in PLoS genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 59 citations in OpenAlex.
- Genetic and Molecular Regulation of Ecdysone Biosynthesis During Insect Metamorphosis.Insects · 2026Review
- HR3/RORα-mediated cholesterol sensing regulates TOR signaling.Nature communications · 2026Article
- miR-184 modulates Ilp8 to control developmental timing during normal growth conditions and in response to developmental perturbations.Development (Cambridge, England) · 2026Article
- Single-cell transcriptomics of theProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Nutrient status alters developmental fates via a switch in mitochondrial homeodynamics.Nature communications · 2025Article
- Article
- Developmental analysis of Spalt function in the Drosophila prothoracic gland.Development (Cambridge, England) · 2024Article
- KDM5-mediated activation of genes required for mitochondrial biology is necessary for viability in Drosophila.Development (Cambridge, England) · 2023Article
- Article
- Genomic Signatures of Recent Adaptation in a Wild Bumblebee.Molecular biology and evolution · 2022Article
- Transcriptional Regulators of Ecdysteroid Biosynthetic Enzymes and Their Roles in Insect Development.Frontiers in physiology · 2022Review
- Activation of EGFR signaling by Tc-Vein and Tc-Spitz regulates the metamorphic transition in the red flour beetle Tribolium castaneum.Scientific reports · 2021Article
- Stop codon readthrough alters the activity of a POU/Oct transcription factor during Drosophila development.BMC biology · 2021Article
- Changes in both trans- and cis-regulatory elements mediate insecticide resistance in a lepidopteron pest, Spodoptera exigua.PLoS genetics · 2021Article
- Regulation of ecdysone production inOpen biology · 2021Review
- Signaling Pathways That Regulate the Crustacean Molting Gland.Frontiers in endocrinology · 2021Review
- Regulation of Body Size and Growth Control.Genetics · 2020Review
- Ecdysone-dependent feedback regulation of prothoracicotropic hormone controls the timing of developmental maturation.Development (Cambridge, England) · 2020Article
- Snail synchronizes endocycling in a TOR-dependent manner to coordinate entry and escape from endoreplication pausing during the Drosophila critical weight checkpoint.PLoS biology · 2020Article
- Poly(A) Binding Protein Is Required for Nuclear Localization of the Ecdysteroidogenic Transcription Factor Molting Defective in the Prothoracic Gland ofFrontiers in genetics · 2020Article
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Authors and funding
10 authors at 6 institutions in 3 countries.
Funding
Abstract
Specialized endocrine cells produce and release steroid hormones that govern development, metabolism and reproduction. In order to synthesize steroids, all the genes in the biosynthetic pathway must be coordinately turned on in steroidogenic cells. In Drosophila, the steroid producing endocrine cells are located in the prothoracic gland (PG) that releases the steroid hormone ecdysone. The transcriptional regulatory network that specifies the unique PG specific expression pattern of the ecdysone biosynthetic genes remains unknown. Here, we show that two transcription factors, the POU-domain Ventral veins lacking (Vvl) and the nuclear receptor Knirps (Kni), have essential roles in the PG during larval development. Vvl is highly expressed in the PG during embryogenesis and is enriched in the gland during larval development, suggesting that Vvl might function as a master transcriptional regulator in this tissue. Vvl and Kni bind to PG specific cis-regulatory elements that are required for expression of the ecdysone biosynthetic genes. Knock down of either vvl or kni in the PG results in a larval developmental arrest due to failure in ecdysone production. Furthermore, Vvl and Kni are also required for maintenance of TOR/S6K and prothoracicotropic hormone (PTTH) signaling in the PG, two major pathways that control ecdysone biosynthesis and PG cell growth. We also show that the transcriptional regulator, Molting defective (Mld), controls early biosynthetic pathway steps. Our data show that Vvl and Kni directly regulate ecdysone biosynthesis by transcriptional control of biosynthetic gene expression and indirectly by affecting PTTH and TOR/S6K signaling. This provides new insight into the regulatory network of transcription factors involved in the coordinated regulation of steroidogenic cell specific transcription, and identifies a new function of Vvl and Knirps in endocrine cells during post-embryonic development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.