ArticleNature protocols2014
Efficient ΦC31 integrase-mediated site-specific germline transformation of Anopheles gambiae.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- CRISPR technologies for the control and study of malaria-transmitting anopheline mosquitoes.Parasites & vectors · 2025Review
- Eliminating malaria vectors with precision-guided sterile males.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Targeting mosquito X-chromosomes reveals complex transmission dynamics of sex ratio distorting gene drives.Nature communications · 2024Article
- CRISPR-mediated germline mutagenesis for genetic sterilization of Anopheles gambiae males.Scientific reports · 2024Article
- Aedes aegypti CCEae3A carboxylase expression confers carbamate, organophosphate and limited pyrethroid resistance in a model transgenic mosquito.PLoS neglected tropical diseases · 2024Article
- Eliminating Malaria Vectors with Precision Guided Sterile Males.bioRxiv : the preprint server for biology · 2023Article
- Neurogenetic identification of mosquito sensory neurons.iScience · 2023Article
- A toolbox of engineered mosquito lines to study salivary gland biology and malaria transmission.PLoS pathogens · 2022Article
- Discrete roles of Ir76b ionotropic coreceptor impact olfaction, blood feeding, and mating in the malaria vector mosquitoProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Multiplexed drug-based selection and counterselection genetic manipulations in Drosophila.Cell reports · 2021Article
- Ammonium transporter AcAmt mutagenesis uncovers reproductive and physiological defects without impacting olfactory responses to ammonia in the malaria vector mosquito Anopheles coluzzii.Insect biochemistry and molecular biology · 2021Article
- Mutagenesis of the orco odorant receptor co-receptor impairs olfactory function in the malaria vector Anopheles coluzzii.Insect biochemistry and molecular biology · 2020Article
- Gene editing reveals obligate and modulatory components of the COInsect biochemistry and molecular biology · 2020Article
- Isolation and transcriptomic analysis ofeLife · 2020Article
- Hemocyte-targeted gene expression in the female malaria mosquito using the hemolectin promoter from Drosophila.Insect biochemistry and molecular biology · 2020Article
- Heterogeneous expression of the ammonium transporter AgAmt in chemosensory appendages of the malaria vector, Anopheles gambiae.Insect biochemistry and molecular biology · 2020Article
- Functional genetic validation of key genes conferring insecticide resistance in the major African malaria vector,Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- A transgenic tool to assess Anopheles mating competitiveness in the field.Parasites & vectors · 2018Article
- Highly Efficient Site-Specific Mutagenesis in Malaria Mosquitoes Using CRISPR.G3 (Bethesda, Md.) · 2018Article
- Tools for Anopheles gambiae Transgenesis.G3 (Bethesda, Md.) · 2015Article
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Authors and funding
8 authors.
Funding
Abstract
Current transgenic methodology developed for mosquitoes has not been applied widely to the major malaria vector Anopheles gambiae, which has proved more difficult to genetically manipulate than other mosquito species and dipteran insects. In this protocol, we describe ΦC31-mediated site-specific integration of transgenes into the genome of A. gambiae. The ΦC31 system has many advantages over 'classical' transposon-mediated germline transformation systems, because it allows integration of large transgenes at specific, characterized genomic locations. Starting from a general protocol, we have optimized steps from embryo collection to co-injection of transgene-containing plasmid and in vitro-produced ΦC31 integrase mRNA. We also provide tips for screening transgenic larvae. The outlined procedure provides robust transformation in A. gambiae, resulting in homozygous transgenic lines in ∼2-3 months.
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