Evidence map›Paper›PMID 24945383›Full record

ArticleNature protocols2014

Isolation and characterization of resident endogenous c-Kit+ cardiac stem cells from the adult mouse and rat heart.

Andrew J Smith, Fiona C Lewis, Iolanda Aquila, Cheryl D Waring, Aurora Nocera, Valter Agosti, Bernardo Nadal-Ginard, Daniele Torella, Georgina M Ellison

Abstract read
PubMed Publisher
In one paragraph

Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.

0numbers the graph read from it
0cells of the map it votes in
68citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

68 citing papers in PubMed.

  1. Review
  2. Article
  3. To Repair a Broken Heart: Stem Cells in Ischemic Heart Disease.Current issues in molecular biology · 2024
    Review
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Review
  10. Review
  11. Article
  12. Article
  13. Article
  14. C-KitIranian journal of basic medical sciences · 2022
    Article
  15. Article
  16. Article
  17. Review
  18. Review
  19. Article
  20. Review

8 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Andrew J Smith1] Centre of Human & Aerospace Physiological Sciences, School of Biomedical Sciences, King's College London, London, UK. [2] Centre for Stem Cells and Regenerative Medicine, King's College London, London, UK.
Fiona C Lewis1] Centre of Human & Aerospace Physiological Sciences, School of Biomedical Sciences, King's College London, London, UK. [2] Centre for Stem Cells and Regenerative Medicine, King's College London, London, UK.
Iolanda AquilaLaboratory of Molecular and Cellular Cardiology, Department of Medical and Surgical Sciences, Magna Graecia University, Catanzaro, Italy.
Cheryl D WaringStem Cell and Regenerative Biology Unit (Biostem), Liverpool John Moores University, Liverpool, UK.
Aurora Nocera1] Laboratory of Molecular Oncology, Department of Experimental and Clinical Medicine, Magna Graecia University, Catanzaro, Italy. [2] CIS for Genomics and Molecular Pathology, Magna Graecia University, Catanzaro, Italy.
Valter Agosti1] Laboratory of Molecular Oncology, Department of Experimental and Clinical Medicine, Magna Graecia University, Catanzaro, Italy. [2] CIS for Genomics and Molecular Pathology, Magna Graecia University, Catanzaro, Italy.
Bernardo Nadal-Ginard1] Centre of Human & Aerospace Physiological Sciences, School of Biomedical Sciences, King's College London, London, UK. [2] Centre for Stem Cells and Regenerative Medicine, King's College London, London, UK.
Daniele Torella1] Laboratory of Molecular and Cellular Cardiology, Department of Medical and Surgical Sciences, Magna Graecia University, Catanzaro, Italy. [2].
Georgina M Ellison1] Centre of Human & Aerospace Physiological Sciences, School of Biomedical Sciences, King's College London, London, UK. [2] Centre for Stem Cells and Regenerative Medicine, King's College London, London, UK. [3] Laboratory of Molecular and Cellular Cardiology, Department of Medical and Surgical Sciences, Magna Graecia University, Catanzaro, Italy. [4].

Funding

British Heart Foundation PG 08/085
6 · The paper itself

Abstract

This protocol describes the isolation of endogenous c-Kit (also known as CD117)-positive (c-Kit(+)), CD45-negative (CD45(-)) cardiac stem cells (eCSCs) from whole adult mouse and rat hearts. The heart is enzymatically digested via retrograde perfusion of the coronary circulation, resulting in rapid and extensive breakdown of the whole heart. Next, the tissue is mechanically dissociated further and cell fractions are separated by centrifugation. The c-Kit(+)CD45(-) eCSC population is isolated by magnetic-activated cell sorting technology and purity and cell numbers are assessed by flow cytometry. This process takes ∼4 h for mouse eCSCs or 4.5 h for rat eCSCs. We also describe how to characterize c-Kit(+)CD45(-) eCSCs. The c-Kit(+)CD45(-) eCSCs exhibit the defining characteristics of stem cells: they are self-renewing, clonogenic and multipotent. This protocol also describes how to differentiate eCSCs into three main cardiac lineages: functional, beating cardiomyocytes, smooth muscle, and endothelial cells. These processes take 17-20 d.

Indexed as

AnimalsCell Culture TechniquesCell DifferentiationCell LineageCells, CulturedMiceMyocardiumProto-Oncogene Proteins c-kitRatsStem CellsProto-Oncogene Proteins c-kit

Identifiers

PMID24945383

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.