Evidence map›Paper›PMID 24943318›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2014

Use of tandem affinity chromatography for purification of cannabinoid receptor CB₂.

Silvia C Locatelli-Hoops, Alexei A Yeliseev

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Recombinant Expression and Purification of Cannabinoid Receptor CBMethods in molecular biology (Clifton, N.J.) · 2021
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Silvia C Locatelli-HoopsLaboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, 5625 Fishers Lane, Bethesda, MD, 20892, USA.
Alexei A Yeliseev
National Institute on Alcohol Abuse and Alcoholism · US

Funding

NMR Investigations Of Cell Membrane StructureZIAAA000003 · NIAAA · NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM · PI GAWRISCH, KLAUS · 2009 to 2020
$23.2M
Intramural NIH HHS Z99 AA999999
6 · The paper itself

Abstract

Tandem affinity purification has been increasingly applied to isolation of recombinant proteins. It relies on two consecutive chromatographic steps that take advantage of the affinity tags placed at opposing ends of the target protein. This allows for efficient removal of contaminating proteins, including products of proteolytic degradation of the fusion that lack either N- or C-terminal tags. Here, we describe the use of two small affinity tags, a poly-histidine tag and a Strep-tag for expression and purification of the human cannabinoid receptor CB2, an integral membrane G protein-coupled receptor.

Indexed as

Amino Acid SequenceChromatography, AffinityEscherichia coliHistidineHumansMolecular BiologyOligopeptidesReceptor, Cannabinoid, CB2Ala-Trp-Arg-His-Pro-Gln-Phe-Gly-GlyHistidineOligopeptidespolyhistidineReceptor, Cannabinoid, CB2

Identifiers

PMID24943318
PMCPMC4784266
OpenAlexW2417422315

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.