Evidence map›Paper›PMID 24942855›Full record

ArticleSmall (Weinheim an der Bergstrasse, Germany)2014

Fracture-based fabrication of normally closed, adjustable, and fully reversible microscale fluidic channels.

Byoung Choul Kim, Christopher Moraes, Jiexi Huang, Toshiki Matsuoka, M D Thouless, Shuichi Takayama

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Small (Weinheim an der Bergstrasse, Germany), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Review
  3. Lithography Technology for Micro- and Nanofabrication.Advances in experimental medicine and biology · 2021
    Article
  4. Article
  5. Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Byoung Choul Kim *Department of Biomedical Engineering, College of Engineering, University of Michigan, 2200 Bonisteel Blvd, Ann Arbor, MI 48109, USA.
Christopher Moraes *Department of Biomedical Engineering, College of Engineering, University of Michigan, 2200 Bonisteel Blvd, Ann Arbor, MI 48109, USA.
Jiexi HuangDepartment of Mechanical Engineering, College of Engineering, University of Michigan, 2350 Hayward St., Ann Arbor, MI 48109, USA.
Toshiki MatsuokaDepartment of Biomedical Engineering, College of Engineering, University of Michigan, 2200 Bonisteel Blvd, Ann Arbor, MI 48109, USA.
M D ThoulessDepartment of Mechanical Engineering, College of Engineering, University of Michigan, 2350 Hayward St., Ann Arbor, MI 48109, USA.
Shuichi TakayamaDepartment of Biomedical Engineering, College of Engineering, University of Michigan, 2200 Bonisteel Blvd, Ann Arbor, MI 48109, USA.

Funding

Active Nanofluidics for Analysis of Chromatin and Genomic DNA StructuresR01HG004653 · NHGRI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI TAKAYAMA, SHUICHI · 2008 to 2010
$1.3M
NHGRI NIH HHS HG004653-03NHGRI NIH HHS R01 HG004653
6 · The paper itself

Abstract

Adjustable fluidic structures play an important role in microfluidic systems. Fracture of multilayered materials under applied tension has been previously demonstrated as a convenient, simple, and inexpensive approach to fabricate nanoscale adjustable structures; here, it is demonstrated how to extend this concept to the microscale. This is achieved by a novel pairing of materials that leverages fracture mechanics to limit crack formation to a specified region, allowing to create size-controllable and adjustable microfluidic structures. This technique can be used to fabricate "normally closed" microfluidic channels that are completely reversible, a feature that is challenging to achieve in conventional systems without careful engineering controls. The adjustable microfluidic channels are then applied to mechanically lyse single cells, and subsequently manipulate the released nuclear chromatin, creating new possibilities for epigenetic analysis of single cells. This simple, versatile, and robust technology provides an easily accessible pathway to construct adjustable microfluidic structures, which will be useful in developing complex assays and experiments even in resource-limited settings.

Indexed as

Microfluidic Analytical TechniquesAnimalsBiocompatible MaterialsCell NucleusChromatinCompressive StrengthDimethylpolysiloxanesEpigenesis, GeneticMaterials TestingMiceMicrofluidicsNanostructuresNanotechnologyNIH 3T3 CellsOxygenPolymersbaysilonBiocompatible MaterialsChromatinDimethylpolysiloxanesOxygenPolymerscracksdynamicsfracturesmicrofluidicsreversibility

Identifiers

PMID24942855
PMCPMC4192030

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.