ArticleMolecular microbiology2014
Adaptor bypass mutations of Bacillus subtilis spx suggest a mechanism for YjbH-enhanced proteolysis of the regulator Spx by ClpXP.
Article in Molecular microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 29 citations in OpenAlex.
- Cell splitting in Staphylococcus aureus is controlled by an adaptor protein facilitating degradation of a peptidoglycan hydrolase.PLoS genetics · 2025Article
- The known unknowns of the Hsp90 chaperone.eLife · 2024Review
- Article
- Structural basis of transcription activation by the global regulator Spx.Nucleic acids research · 2021Article
- The Division Defect of aJournal of bacteriology · 2021Article
- Regulatory circuits controlling Spx levels in Streptococcus mutans.Molecular microbiology · 2020Article
- YjbH Requires Its Thioredoxin Active Motif for the Nitrosative Stress Response, Cell-to-Cell Spread, and Protein-Protein Interactions in Listeria monocytogenes.Journal of bacteriology · 2020Article
- Role of Regulated Proteolysis in the Communication of Bacteria With the Environment.Frontiers in molecular biosciences · 2020Review
- Protein aggregation in bacteria.FEMS microbiology reviews · 2020Review
- YjbH Solubility Controls Spx inFrontiers in microbiology · 2020Article
- Identification of Novel Spx Regulatory Pathways in Bacillus subtilis Uncovers a Close Relationship between the CtsR and Spx Regulons.Journal of bacteriology · 2019Article
- Roles and regulation of Spx family transcription factors in Bacillus subtilis and related species.Advances in microbial physiology · 2019Review
- Stabilization of Bacillus subtilis Spx under cell wall stress requires the anti-adaptor protein YirB.PLoS genetics · 2018Article
- Regulated Proteolysis in Bacteria.Annual review of biochemistry · 2018Review
- The C-Terminal Region of Bacillus subtilis SwrA Is Required for Activity and Adaptor-Dependent LonA Proteolysis.Journal of bacteriology · 2018Article
- Cargo engagement protects protease adaptors from degradation in a substrate-specific manner.The Journal of biological chemistry · 2017Article
- Selective adaptor dependent protein degradation in bacteria.Current opinion in microbiology · 2017Review
- Functional Diversity of AAA+ Protease Complexes inFrontiers in molecular biosciences · 2017Review
- Regulated Proteolysis in Bacteria: Caulobacter.Annual review of genetics · 2016Review
- Rifampin Resistance rpoB Alleles or Multicopy Thioredoxin/Thioredoxin Reductase Suppresses the Lethality of Disruption of the Global Stress Regulator spx in Staphylococcus aureus.Journal of bacteriology · 2016Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The global regulator, Spx, is under proteolytic control exerted by the adaptor YjbH and ATP-dependent protease ClpXP in Bacillus subtilis. While YjbH is observed to bind the Spx C-terminus, YjbH shows little affinity for ClpXP, indicating adaptor activity that does not operate by tethering. Chimeric proteins derived from B. subtilis AbrB and the Spx C-terminus showed that a 28-residue C-terminal section of Spx (AbrB28), but not the last 12 or 16 residues (AbrB12, AbrB16), was required for YjbH interaction and for ClpXP proteolysis, although the rate of AbrB28 proteolysis was not affected by YjbH addition. The result suggested that the YjbH-targeted 28 residue segment of the Spx C-terminus bears a ClpXP-recognition element(s) that is hidden in the intact Spx protein. Residue substitutions in the conserved helix α6 of the C-terminal region generated Spx substrates that were degraded by ClpXP at accelerated rates compared to wild-type Spx, and showed reduced dependency on the YjbH activity. The residue substitutions also weakened the interaction between Spx and YjbH. The results suggest a model in which YjbH, through interaction with residues of helix α6, exposes the C-terminus of Spx for recognition and proteolysis by ClpXP.
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