ArticleRNA (New York, N.Y.)2014
A Spinach molecular beacon triggered by strand displacement.
Article in RNA (New York, N.Y.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 62 citations in OpenAlex.
- A selection pipeline for switchable aptamer beacons in broad-spectrum protein detection.Nature biomedical engineering · 2026Article
- Compact RNA sensors for increasingly complex functions of multiple inputs.Nature chemistry · 2025Article
- Programmable fluorescent aptamer-based RNA switches for rapid identification of point mutations.Nature chemistry · 2025Article
- Structure-based insights into fluorogenic RNA aptamers.Acta biochimica et biophysica Sinica · 2024Review
- Article
- An RNA origami robot that traps and releases a fluorescent aptamer.Science advances · 2024Article
- Turn-on RNA Mango Beacons forRNA (New York, N.Y.) · 2024Article
- DNA Logic Gates for Small Molecule Activation Circuits in Cells.ACS synthetic biology · 2024Article
- Multi-arm RNA junctions encoding molecular logic unconstrained by input sequence for versatile cell-free diagnostics.Nature biomedical engineering · 2022Article
- Electrophoretic mobility shift as a molecular beacon-based readout for miRNA detection.Biosensors & bioelectronics · 2021Article
- Splitting aptamers and nucleic acid enzymes for the development of advanced biosensors.Nucleic acids research · 2020Review
- Article
- Self-powered RNA nanomachine driven by metastable structure.Nucleic acids research · 2019Article
- Structural basis for activation of fluorogenic dyes by an RNA aptamer lacking a G-quadruplex motif.Nature communications · 2018Article
- Genetically Encoded Catalytic Hairpin Assembly for Sensitive RNA Imaging in Live Cells.Journal of the American Chemical Society · 2018Article
- Enzyme-free ultrasensitive fluorescence detection of epithelial cell adhesion molecules based on a toehold-aided DNA recycling amplification strategy.RSC advances · 2018Article
- Light-Up RNA Aptamers and Their Cognate Fluorogens: From Their Development to Their Applications.International journal of molecular sciences · 2017Review
- Structural Principles of Fluorescent RNA Aptamers.Trends in pharmacological sciences · 2017Review
- Divide and Control: Comparison of Split and Switch Hybridization Sensors.ChemistrySelect · 2017Article
- Split Spinach Aptamer for Highly Selective Recognition of DNA and RNA at Ambient Temperatures.Chembiochem : a European journal of chemical biology · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We have re-engineered the fluorescent RNA aptamer Spinach to be activated in a sequence-dependent manner. The original Spinach aptamer was extended at its 5'- and 3'-ends to create Spinach.ST, which is predicted to fold into an inactive conformation and thus prevent association with the small molecule fluorophore DFHBI. Hybridization of a specific trigger oligonucleotide to a designed toehold leads to toehold-initiated strand displacement and refolds Spinach into the active, fluorophore-binding conformation. Spinach.ST not only specifically detects its target oligonucleotide but can discriminate readily against single-nucleotide mismatches. RNA amplicons produced during nucleic acid sequence-based amplification (NASBA) of DNA or RNA targets could be specifically detected and reported in real-time by conformational activation of Spinach.ST generated by in vitro transcription. In order to adapt any target sequence to detection by a Spinach reporter we used a primer design technique that brings together otherwise distal toehold sequences via hairpin formation. The same techniques could potentially be used to adapt common Spinach reporters to non-nucleic acid analytes, rather than by making fusions between aptamers and Spinach.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.