ArticlePathogens and disease2014
Biofilm-specific extracellular matrix proteins of nontypeable Haemophilus influenzae.
Article in Pathogens and disease, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
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- OprF Impacts Pseudomonas aeruginosa Biofilm Matrix eDNA Levels in a Nutrient-Dependent Manner.Journal of bacteriology · 2023Article
- The alginate polymer OligoG alters susceptibility of biofilm-embedded non-typeableJAC-antimicrobial resistance · 2023Article
- Extracellular matrix remodeling in tumor progression and immune escape: from mechanisms to treatments.Molecular cancer · 2023Review
- OprF impactsbioRxiv : the preprint server for biology · 2023Article
- Biofilm Signaling, Composition and Regulation inJournal of microbiology and biotechnology · 2023Review
- Hydrodynamic conditions affect the proteomic profile of marine biofilms formed by filamentous cyanobacterium.NPJ biofilms and microbiomes · 2022Article
- Protein domain-dependent vesiculation of Lipoprotein A, a protein that is important in cell wall synthesis and fitness of the human respiratory pathogenFrontiers in cellular and infection microbiology · 2022Article
- Superhydrophobic Nanocoatings as Intervention against Biofilm-Associated Bacterial Infections.Nanomaterials (Basel, Switzerland) · 2021Review
- The Role of Non-TypeableFrontiers in cellular and infection microbiology · 2021Review
- Analytical combinations to evaluate the macromolecular composition of extracellular substances (ECS) from Lactobacillus plantarum cell culture media.Analytical and bioanalytical chemistry · 2021Article
- Slr4, a newly identified S-layer protein from marine Gammaproteobacteria, is a major biofilm matrix component.Molecular microbiology · 2020Article
- Characteristics of monolayer formationBiofilm · 2019Article
- Detection ofJournal of clinical and experimental dentistry · 2019Article
- Biofilm biology and vaccine strategies for otitis media due to nontypeableJournal of pediatric infectious diseases · 2019Article
- Modulation of S. aureus and P. aeruginosa biofilm: an in vitro study with new coumarin derivatives.World journal of microbiology & biotechnology · 2018Article
- Options and Limitations in Clinical Investigation of Bacterial Biofilms.Clinical microbiology reviews · 2018Review
- Article
- Sterilization of Biofilm on a Titanium Surface Using a Combination of Nonthermal Plasma and Chlorhexidine Digluconate.BioMed research international · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 7 institutions in 3 countries.
Funding
Abstract
Nontypeable Haemophilus influenzae (NTHi), a human respiratory tract pathogen, can form colony biofilms in vitro. Bacterial cells and the amorphous extracellular matrix (ECM) constituting the biofilm can be separated using sonication. The ECM from 24- and 96-h NTHi biofilms contained polysaccharides and proteinaceous components as detected by nuclear magnetic resonance (NMR) and Fourier transform infrared spectroscopy (FTIR) spectroscopy. More conventional chemical assays on the biofilm ECM confirmed the presence of these components and also DNA. Proteomics revealed eighteen proteins present in biofilm ECM that were not detected in planktonic bacteria. One ECM protein was unique to 24-h biofilms, two were found only in 96-h biofilms, and fifteen were present in the ECM of both 24- and 96-h NTHi biofilms. All proteins identified were either associated with bacterial membranes or cytoplasmic proteins. Immunocytochemistry showed two of the identified proteins, a DNA-directed RNA polymerase and the outer membrane protein OMP P2, associated with bacteria and biofilm ECM. Identification of biofilm-specific proteins present in immature biofilms is an important step in understanding the in vitro process of NTHi biofilm formation. The presence of a cytoplasmic protein and a membrane protein in the biofilm ECM of immature NTHi biofilms suggests that bacterial cell lysis may be a feature of early biofilm formation.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.