ArticleNature communications2014
Vanillin formation from ferulic acid in Vanilla planifolia is catalysed by a single enzyme.
Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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64 citing papers in PubMed, 226 citations in OpenAlex.
- Plant metabolic adaptation to nitrogen scarcity: the role of biological nitrification inhibitors.Essays in biochemistry · 2026Review
- Efficient Synthesis of Glucovanillin and Elucidation of Its Molecular Mechanisms in Ameliorating T2DM via Core Target Modulation and α-Glucosidase Inhibition.Molecules (Basel, Switzerland) · 2026Article
- Genome-wide identification and expression profiling of theFrontiers in plant science · 2026Article
- Vanillin Beyond Flavor: Therapeutic Potentials and Emerging Applications in Hydrogel-Based Biomaterials.Gels (Basel, Switzerland) · 2025Review
- Advanced recovery of bioactive polyphenols: integrated optimization of drying and green extraction for avocado seed by-products.Food chemistry: X · 2025Article
- Unravelling Vanillin Biosynthesis: Integrative Transcriptomic and Metabolomic Insights intoJournal of agricultural and food chemistry · 2025Article
- Biosilicification in monocots: Comparative analysis highlights contrasting patterns of deposition.American journal of botany · 2025Article
- Time-Series Metabolome and Transcriptome Analyses Reveal the Genetic Basis of Vanillin Biosynthesis in Vanilla.Plants (Basel, Switzerland) · 2025Article
- Regulating Sirtuin 3-mediated mitochondrial dynamics through vanillic acid improves muscle atrophy in cancer-induced cachexia.Communications biology · 2025Article
- Comparative transcriptome profiling of vanilla (Vanilla planifolia) capsule development provides insights of vanillin biosynthesis.BMC plant biology · 2025Article
- Comprehensive metabolome characterization of leaves, internodes, and aerial roots of Vanilla planifolia by untargeted LC-MS and GC × GC-MS.Phytochemical analysis : PCA · 2025Article
- Genetic Engineering Approaches for the Microbial Production of Vanillin.Biomolecules · 2024Review
- Floral volatile benzenoids/phenylpropanoids: biosynthetic pathway, regulation and ecological value.Horticulture research · 2024Article
- Exploring Water-Soluble South AfricanCurrent issues in molecular biology · 2024Article
- Engineering a coenzyme-independent dioxygenase for one-step production of vanillin from ferulic acid.Applied and environmental microbiology · 2024Article
- Minimal aromatic aldehyde reduction (MARE) yeast platform for engineering vanillin production.Biotechnology for biofuels and bioproducts · 2024Article
- Bacterial transformation of lignin: key enzymes and high-value products.Biotechnology for biofuels and bioproducts · 2024Review
- The postbiotic potential ofFrontiers in microbiology · 2024Review
- Metabolic engineering of hairy root cultures inFrontiers in bioengineering and biotechnology · 2024Article
- Identification of ethyl vanillin in strawberry (Fragaria × ananassa) using a targeted metabolomics strategy: From artificial to natural.Food chemistry: X · 2023Article
4 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 3 institutions in 3 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Vanillin is a popular and valuable flavour compound. It is the key constituent of the natural vanilla flavour obtained from cured vanilla pods. Here we show that a single hydratase/lyase type enzyme designated vanillin synthase (VpVAN) catalyses direct conversion of ferulic acid and its glucoside into vanillin and its glucoside, respectively. The enzyme shows high sequence similarity to cysteine proteinases and is specific to the substitution pattern at the aromatic ring and does not metabolize caffeic acid and p-coumaric acid as demonstrated by coupled transcription/translation assays. VpVAN localizes to the inner part of the vanilla pod and high transcript levels are found in single cells located a few cell layers from the inner epidermis. Transient expression of VpVAN in tobacco and stable expression in barley in combination with the action of endogenous alcohol dehydrogenases and UDP-glucosyltransferases result in vanillyl alcohol glucoside formation from endogenous ferulic acid. A gene encoding an enzyme showing 71% sequence identity to VpVAN was identified in another vanillin-producing plant species Glechoma hederacea and was also shown to be a vanillin synthase as demonstrated by transient expression in tobacco.
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