ArticleScientific reports2014
Light-induced rapid Ca²⁺ response and MAPK phosphorylation in the cells heterologously expressing human OPN5.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- Non-visual opsins and their role in circadian photoentrainment.Eye and vision (London, England) · 2026Review
- The physiological functions of OPN5m.Eye and vision (London, England) · 2025Review
- Article
- Direct Stimulation of Gastric Smooth Muscle Cells via GNeurogastroenterology and motility · 2025Article
- Expression and Mutation ofThe journal of poultry science · 2024Article
- Network-driven intracellular cAMP coordinates circadian rhythm in the suprachiasmatic nucleus.Science advances · 2023Article
- Selective optogenetic control of GNature communications · 2022Article
- Ocular and extraocular roles of neuropsin in vertebrates.Trends in neurosciences · 2022Review
- Non-visual Opsins and Novel Photo-Detectors in the Vertebrate Inner Retina Mediate Light Responses Within the Blue Spectrum Region.Cellular and molecular neurobiology · 2022Review
- Opsin 5 is a key regulator of ultraviolet radiation-induced melanogenesis in human epidermal melanocytes.The British journal of dermatology · 2021Article
- Neuropsin (OPN5) Mediates Local Light-Dependent Induction of Circadian Clock Genes and Circadian Photoentrainment in Exposed Murine Skin.Current biology : CB · 2019Article
- Localization of the ultraviolet-sensor Opn5m and its effect on myopia-related gene expression in the late-embryonic chick eye.Biochemistry and biophysics reports · 2019Article
- Expression of Non-visual Opsins Opn3 and Opn5 in the Developing Inner Retinal Cells of Birds. Light-Responses in Müller Glial Cells.Frontiers in cellular neuroscience · 2019Article
- Article
- Neuropsin (OPN5)-mediated photoentrainment of local circadian oscillators in mammalian retina and cornea.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Combining fluorescence and bioluminescence microscopy.Microscopy research and technique · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular imaging is a powerful tool for investigating intracellular signalling, but it is difficult to acquire conventional fluorescence imaging from photoreceptive cells. Here we demonstrated that human opsin5 (OPN5) photoreceptor mediates light-induced Ca(2+) response in human embryonic kidney (HEK293) and mouse neuroblastoma (Neuro2a) cell lines using a luminescence imaging system with a fluorescent indicator and a newly synthesized bioluminescent indicator. Weak light fluorescence and bioluminescence imaging revealed rapid and transient light-stimulated Ca(2+) release from thapsigargin-sensitive Ca(2+) stores, whereas long-lasting Ca(2+) elevation was observed using a conventional fluorescence imaging system. Bioluminescence imaging also demonstrated that OPN5 activation in HEK293 cells induced a decrease in pertussis toxin-sensitive cAMP, confirming previous reports. In addition, ultraviolet radiation induced the phosphorylation of mitogen-activated protein kinases when OPN5 was stimulated in Neuro2a cells. These findings suggest that the combination of these imaging approaches may provide a new means to investigate the physiological characteristics of photoreceptors.
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