ArticlePloS one2014
Differential acetylation of histone H3 at the regulatory region of OsDREB1b promoter facilitates chromatin remodelling and transcription activation during cold stress.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 38 papers.
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38 citing papers in PubMed, 96 citations in OpenAlex.
- Epigenetic Control of Cold Stress Tolerance in Plants: Emerging Mechanisms and Applications for Crop Improvement.International journal of molecular sciences · 2026Review
- Histone acetyltransferases as integrative epigenetic regulators of plant abiotic stress responses.Plant cell reports · 2026Review
- A nuclear CobW/WW-domain factor represses the COProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Analysis of Stress Response Genes in Microtuberization of PotatoPlants (Basel, Switzerland) · 2024Article
- Molecular Mechanisms Underlying Freezing Tolerance in Plants: Implications for Cryopreservation.International journal of molecular sciences · 2024Review
- Plant responses to abiotic stress regulated by histone acetylation.Frontiers in plant science · 2024Review
- Regulation of reactive oxygen molecules in pakchoi by histone acetylation modifications under Cd stress.PloS one · 2024Article
- Research progress on the physiological response and molecular mechanism of cold response in plants.Frontiers in plant science · 2024Review
- Transcriptional and Metabolic Profiling ofMetabolites · 2023Article
- LncRNAs elevate plant adaptation under low temperature by maintaining local chromatin landscape.Plant signaling & behavior · 2022Review
- Molecular and Physiological Mechanisms to Mitigate Abiotic Stress Conditions in Plants.Life (Basel, Switzerland) · 2022Review
- Chromatin-Based Transcriptional Reprogramming in Plants under Abiotic Stresses.Plants (Basel, Switzerland) · 2022Review
- Genetic Mechanisms of Cold Signaling in Wheat (Life (Basel, Switzerland) · 2022Review
- Stochastic Variation in DNA Methylation Modulates Nucleosome Occupancy and Alternative Splicing inPlants (Basel, Switzerland) · 2022Article
- Review
- Review
- Multifaceted Chromatin Structure and Transcription Changes in Plant Stress Response.International journal of molecular sciences · 2021Review
- NAD metabolism in aging and cancer.Experimental biology and medicine (Maywood, N.J.) · 2020Article
- Transcriptomic profiling of germinating seeds under cold stress and characterization of the cold-tolerant gene LTG5 in rice.BMC plant biology · 2020Article
- Understanding the early cold response mechanism in IR64 indica rice variety through comparative transcriptome analysis.BMC genomics · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rice ortholog of DREB1, OsDREB1b, is transcriptionally induced by cold stress and over-expression of OsDREB1b results in increase tolerance towards high salt and freezing stress. This spatio-temporal expression of OsDREB1b is preceded by the change in chromatin structure at the promoter and the upstream region for gene activation. The promoter and the upstream region of OsDREB1b genes appear to be arranged into a nucleosome array. Nucleosome mapping of ∼ 700 bp upstream region of OsDREB1b shows two positioned nucleosomes between -610 to -258 and a weakly positioned nucleosome at the core promoter and the TSS. Upon cold stress, there is a significant change in the nucleosome arrangement at the upstream region with increase in DNaseI hypersensitivity or MNase digestion in the vicinity of cis elements and TATA box at the core promoter. ChIP assays shows hyper-acetylation of histone H3K9 throughout the locus whereas region specific increase was observed in H3K14ac and H3K27ac. Moreover, there is an enrichment of RNA PolII occupancy at the promoter region during transcription activation. There is no significant change in the H3 occupancy in OsDREB1b locus negating the possibility of nucleosome loss during cold stress. Interestingly, cold induced enhanced transcript level of OsDREB1b as well as histone H3 acetylation at the upstream region was found to diminish when stressed plants were returned to normal temperature. The result indicates absolute necessity of changes in chromatin conformation for the transcription up-regulation of OsDREB1b gene in response to cold stress. The combined results show the existence of closed chromatin conformation at the upstream and promoter region of OsDREB1b in the transcription "off" state. During cold stress, changes in region specific histone modification marks promote the alteration of chromatin structure to facilitate the binding of transcription machinery for proper gene expression.
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