ArticleBiophysical journal2014
N-nervonoylsphingomyelin (C24:1) prevents lateral heterogeneity in cholesterol-containing membranes.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 57 citations in OpenAlex.
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- Heptanol-mediated phase separation determines phase preference of molecules in live cell membranes.Journal of lipid research · 2022Article
- Formation and Nanoscale Characterization of Asymmetric Supported Lipid Bilayers Containing Raft-Like Domains.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Chemically synthesized GbEuropean biophysics journal : EBJ · 2021Review
- Sphingomyelin Acyl Chains Influence the Formation of Sphingomyelin- and Cholesterol-Enriched Domains.Biophysical journal · 2020Article
- Multiple Sclerosis: Lipids, Lymphocytes, and Vitamin D.Immunometabolism · 2020Article
- Synthesis of GbAngewandte Chemie (International ed. in English) · 2019Article
- Complex Phase Behavior of GUVs Containing Different Sphingomyelins.Biophysical journal · 2019Article
- Does Ceramide Form Channels? The Ceramide-Induced Membrane Permeabilization Mechanism.Biophysical journal · 2017Article
- Mechanisms by Which Pleiotropic AmphiphilicCurrent colorectal cancer reports · 2014Article
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study was conducted to explore how the nature of the acyl chains of sphingomyelin (SM) influence its lateral distribution in the ternary lipid mixture SM/cholesterol/1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), focusing on the importance of the hydrophobic part of the SM molecule for domain formation. Atomic force microscopy (AFM) measurements showed that the presence of a double bond in the 24:1 SM molecule in mixtures with cholesterol (CHO) or in pure bilayers led to a decrease in the molecular packing. Confocal microscopy and AFM showed, at the meso- and nanoscales respectively, that unlike 16:0 and 24:0 SM, 24:1 SM does not induce phase segregation in ternary lipid mixtures with DOPC and CHO. This ternary lipid mixture had a nanomechanical stability intermediate between those displayed by liquid-ordered (Lo) and liquid-disordered (Ld) phases, as reported by AFM force spectroscopy measurements, demonstrating that 24:1 SM is able to accommodate both DOPC and CHO, forming a single phase. Confocal experiments on giant unilamellar vesicles made of human, sheep, and rabbit erythrocyte ghosts rich in 24:1 SM and CHO, showed no lateral domain segregation. This study provides insights into how the specific molecular structure of SM affects the lateral behavior and the physical properties of both model and natural membranes. Specifically, the data suggest that unsaturated SM may help to keep membrane lipids in a homogeneous mixture rather than in separate domains.
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