ArticleBiophysical journal2014
Sodium and proton effects on inward proton transport through Na/K pumps.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- On the mechanism of hypomagnesemia with treatment-resistant seizures caused by variants of the Na+,K+-ATPase α1 subunit (ATP1A1).The Journal of general physiology · 2026Article
- Structure and function of HNature communications · 2022Article
- Diseases caused by mutations in the NaAmerican journal of physiology. Cell physiology · 2021Review
- Distinct effects of Q925 mutation on intracellular and extracellular NaScientific reports · 2019Article
- Na/K Pump Mutations Associated with Primary Hyperaldosteronism Cause Loss of Function.Biochemistry · 2019Article
- Functional consequences of the CAPOS mutation E818K of NaThe Journal of biological chemistry · 2019Article
- A novel ATP1A2 mutation in a patient with hypokalaemic periodic paralysis and CNS symptoms.Brain : a journal of neurology · 2018Article
- External Ion Access in the Na/K Pump: Kinetics of NaBiophysical journal · 2018Article
- On the effect of hyperaldosteronism-inducing mutations in Na/K pumps.The Journal of general physiology · 2017Article
- Tracking protein function with sodium multi quantum spectroscopy in a 3D-tissue culture based on microcavity arrays.Scientific reports · 2017Article
- Arginine substitution of a cysteine in transmembrane helix M8 converts Na+,K+-ATPase to an electroneutral pump similar to H+,K+-ATPase.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Intracellular Requirements for Passive Proton Transport through the NaBiophysical journal · 2016Article
- The Inner Workings of Proton Slippage through the Sodium Pump.Biophysical journal · 2016Article
- Article
- ATP1A2 Mutations in Migraine: Seeing through the Facets of an Ion Pump onto the Neurobiology of Disease.Frontiers in physiology · 2016Review
- Importance of the Voltage Dependence of Cardiac Na/K ATPase Isozymes.Biophysical journal · 2015Article
- Does the sodium pump have secret levels?Biophysical journal · 2014Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The Na/K pump hydrolyzes ATP to export three intracellular Na (Nai) as it imports two extracellular K (Ko) across animal plasma membranes. Within the protein, two ion-binding sites (sites I and II) can reciprocally bind Na or K, but a third site (site III) exclusively binds Na in a voltage-dependent fashion. In the absence of Nao and Ko, the pump passively imports protons, generating an inward current (IH). To elucidate the mechanisms of IH, we used voltage-clamp techniques to investigate the [H]o, [Na]o, and voltage dependence of IH in Na/K pumps from ventricular myocytes and in ouabain-resistant pumps expressed in Xenopus oocytes. Lowering pHo revealed that Ho both activates IH (in a voltage-dependent manner) and inhibits it (in a voltage-independent manner) by binding to different sites. Nao effects depend on pHo; at pHo where no Ho inhibition is observed, Nao inhibits IH at all concentrations, but when applied at pHo that inhibits pump-mediated current, low [Na]o activates IH and high [Na]o inhibits it. Our results demonstrate that IH is a property inherent to Na/K pumps, not linked to the oocyte expression environment, explains differences in the characteristics of IH previously reported in the literature, and supports a model in which 1), protons leak through site III; 2), binding of two Na or two protons to sites I and II inhibits proton transport; and 3), pumps with mixed Na/proton occupancy of sites I and II remain permeable to protons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.