ArticleBiomedical optics express2014
Double nanohole optical tweezers visualize protein p53 suppressing unzipping of single DNA-hairpins.
Article in Biomedical optics express, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 87 citations in OpenAlex.
- Label-free optical observation of disordered-to-ordered transitions in single intrinsically disordered proteins.Npj biosensing · 2026Article
- Optical tweezers in biomedical research - progress and techniques.Journal of medicine and life · 2024Review
- Influence of point mutations on PR65 conformational adaptability: Insights from molecular simulations and nanoaperture optical tweezers.Science advances · 2024Article
- Recent Advancements in Nanophotonics for Optofluidics.Advances in physics: X · 2024Article
- Plasmonic Bowl-Shaped Nanopore for Raman Detection of Single DNA Molecules in Flow-Through.Nano letters · 2023Article
- Label-Free Optical Analysis of Biomolecules in Solid-State Nanopores: Toward Single-Molecule Protein Sequencing.ACS photonics · 2022Review
- Modelling of the dynamic polarizability of macromolecules for single-molecule optical biosensing.Scientific reports · 2022Article
- Single-molecule kinetic locking allows fluorescence-free quantification of protein/nucleic-acid binding.Communications biology · 2021Article
- Enhancing Single-Molecule Fluorescence Spectroscopy with Simple and Robust Hybrid Nanoapertures.ACS photonics · 2021Article
- Plasmonic tweezers: for nanoscale optical trapping and beyond.Light, science & applications · 2021Review
- Overcoming Diffusion-Limited Trapping in Nanoaperture Tweezers Using Opto-Thermal-Induced Flow.Nano letters · 2020Article
- Applications of Optically Controlled Gold Nanostructures in Biomedical Engineering.Frontiers in bioengineering and biotechnology · 2020Review
- Towards biological applications of nanophotonic tweezers.Current opinion in chemical biology · 2019Review
- Bio-Molecular Applications of Recent Developments in Optical Tweezers.Biomolecules · 2019Review
- Article
- Nanophotonic trapping: precise manipulation and measurement of biomolecular arrays.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2018Review
- Photonic-plasmonic hybrid single-molecule nanosensor measures the effect of fluorescent labels on DNA-protein dynamics.Science advances · 2017Article
- Characterization of the near-field and convectional transport behavior of micro and nanoparticles in nanoscale plasmonic optical lattices.Biomicrofluidics · 2016Article
- Get to Understand More from Single-Cells: Current Studies of Microfluidic-Based Techniques for Single-Cell Analysis.International journal of molecular sciences · 2015Review
- Origin and Future of Plasmonic Optical Tweezers.Nanomaterials (Basel, Switzerland) · 2015Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we report on the use of double-nanohole (DNH) optical tweezers as a label-free and free-solution single-molecule probe for protein-DNA interactions. Using this approach, we demonstrate the unzipping of individual 10 base pair DNA-hairpins, and quantify how tumor suppressor p53 protein delays the unzipping. From the Arrhenius behavior, we find the energy barrier to unzipping introduced by p53 to be 2 × 10(-20) J, whereas cys135ser mutant p53 does not show suppression of unzipping, which gives clues to its functional inability to suppress tumor growth. This transformative approach to single molecule analysis allows for ultra-sensitive detection and quantification of protein-DNA interactions to revolutionize the fight against genetic diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.