ArticleBiomedical optics express2014
Wavefront sensorless adaptive optics temporal focusing-based multiphoton microscopy.
Article in Biomedical optics express, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Temporal focusing multiphoton microscopy with cross-modality multi-stage 3D U-Net for fast and clear bioimaging.Biomedical optics express · 2023Article
- Temporal focusing multiphoton microscopy with optimized parallel multiline scanning for fast biotissue imaging.Journal of biomedical optics · 2021Article
- Two-photon imaging of the mammalian retina with ultrafast pulsing laser.JCI insight · 2018Article
- Four-dimensional light shaping: manipulating ultrafast spatiotemporal foci in space and time.Light, science & applications · 2018Article
- High-throughput multiphoton-induced three-dimensional ablation and imaging for biotissues.Biomedical optics express · 2015Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Temporal profile distortions reduce excitation efficiency and image quality in temporal focusing-based multiphoton microscopy. In order to compensate the distortions, a wavefront sensorless adaptive optics system (AOS) was integrated into the microscope. The feedback control signal of the AOS was acquired from local image intensity maximization via a hill-climbing algorithm. The control signal was then utilized to drive a deformable mirror in such a way as to eliminate the distortions. With the AOS correction, not only is the axial excitation symmetrically refocused, but the axial resolution with full two-photon excited fluorescence (TPEF) intensity is also maintained. Hence, the contrast of the TPEF image of a R6G-doped PMMA thin film is enhanced along with a 3.7-fold increase in intensity. Furthermore, the TPEF image quality of 1μm fluorescent beads sealed in agarose gel at different depths is improved.
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Registered trials
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