ArticleNucleic acids research2014
Mosquito and Drosophila entomobirnaviruses suppress dsRNA- and siRNA-induced RNAi.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
64 citing papers in PubMed, 100 citations in OpenAlex.
- Exploring the potential of RNA interference (RNAi) in mosquito control: from mechanisms to molecular insights.In vitro cellular & developmental biology. Animal · 2026Review
- A conserved in-frame stop codon acts as a multipotent defense mechanism in alphaviruses.Science advances · 2026Article
- Life stage-dependent RNA interference in the tropical rock lobsterMarine life science & technology · 2026Article
- The Sindbis virus nsP3 opal codon protects viral RNA and fitness by maintaining replication spherule integrity.bioRxiv : the preprint server for biology · 2025Article
- Differential effect of acuteOne health (Amsterdam, Netherlands) · 2025Article
- Deep Sequencing Analysis of Virome Components, Viral Gene Expression and Antiviral RNAi Responses inInternational journal of molecular sciences · 2024Article
- Structure of the aminoterminal domain of the birnaviral multifunctional VP3 protein and its unexplored critical role.PNAS nexus · 2024Article
- Interaction between Bombyx mori Cytoplasmic Polyhedrosis Virus NSP8 and BmAgo2 Inhibits RNA Interference and Enhances Virus Proliferation.Microbiology spectrum · 2023Article
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- BmNPV p35 Reduces the Accumulation of Virus-Derived siRNAs and Hinders the Function of siRNAs to Facilitate Viral Infection.Frontiers in immunology · 2022Article
- Antiviral RNA interference in disease vector (Asian longhorned) ticks.PLoS pathogens · 2021Article
- Innate and adaptive resistance to RNAi: a major challenge and hurdle to the development of double stranded RNA-based pesticides.3 Biotech · 2021Review
- Type I Interferon acts as a major barrier to the establishment of infectious bursal disease virus (IBDV) persistent infections.Journal of virology · 2021Article
- Structure and dsRNA-binding activity of the Birnavirus Drosophila X Virus VP3 protein.Journal of virology · 2021Article
- Symbiotic Interactions Between Mosquitoes and Mosquito Viruses.Frontiers in cellular and infection microbiology · 2021Review
- Mechanisms of Cell Entry by dsRNA Viruses: Insights for Efficient Delivery of dsRNA and Tools for Improved RNAi-Based Pest Control.Frontiers in physiology · 2021Review
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- Discrete Virus Factories Form in the Cytoplasm of Cells Coinfected with Two Replication-Competent Tagged Reporter Birnaviruses That Subsequently Coalesce over Time.Journal of virology · 2020Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference (RNAi) is a crucial antiviral defense mechanism in insects, including the major mosquito species that transmit important human viruses. To counteract the potent antiviral RNAi pathway, insect viruses encode RNAi suppressors. However, whether mosquito-specific viruses suppress RNAi remains unclear. We therefore set out to study RNAi suppression by Culex Y virus (CYV), a mosquito-specific virus of the Birnaviridae family that was recently isolated from Culex pipiens mosquitoes. We found that the Culex RNAi machinery processes CYV double-stranded RNA (dsRNA) into viral small interfering RNAs (vsiRNAs). Furthermore, we show that RNAi is suppressed in CYV-infected cells and that the viral VP3 protein is responsible for RNAi antagonism. We demonstrate that VP3 can functionally replace B2, the well-characterized RNAi suppressor of Flock House virus. VP3 was found to bind long dsRNA as well as siRNAs and interfered with Dicer-2-mediated cleavage of long dsRNA into siRNAs. Slicing of target RNAs by pre-assembled RNA-induced silencing complexes was not affected by VP3. Finally, we show that the RNAi-suppressive activity of VP3 is conserved in Drosophila X virus, a birnavirus that persistently infects Drosophila cell cultures. Together, our data indicate that mosquito-specific viruses may encode RNAi antagonists to suppress antiviral RNAi.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.