Evidence map›Paper›PMID 24939889›Full record

ArticlemBio2014

Standards for sequencing viral genomes in the era of high-throughput sequencing.

Jason T Ladner, Brett Beitzel, Patrick S G Chain, Matthew G Davenport, Eric F Donaldson, Matthew Frieman, Jeffrey R Kugelman, Jens H Kuhn, Jules O'Rear, Pardis C Sabeti and 7 more

Open access · goldAbstract readEditorial
In one paragraph

Article in mBio, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.

0numbers the graph read from it
0cells of the map it votes in
65citing papers in PubMed
16.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

65 citing papers in PubMed, 157 citations in OpenAlex.

  1. Article
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  7. Detection of dengue virus serotype 4 in Panama after 23 years without circulation.Frontiers in cellular and infection microbiology · 2024
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5 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

17 authors at 9 institutions in 1 country.

Jason T LadnerCenter for Genome Sciences, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA jason.t.ladner.ctr@mail.mil gustavo.f.palacios.ctr@mail.mil.
Brett BeitzelCenter for Genome Sciences, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA.
Patrick S G ChainBioinformatics and Analytics Team, Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico, USA.
Matthew G DavenportNational Security Systems Biology Center, Asymmetric Operations Sector, Johns Hopkins University, Applied Physics Laboratory, Laurel, Maryland, USA.
Eric F DonaldsonU.S. Food and Drug Administration, Silver Spring, Maryland, USA Filovirus Animal Nonclinical Group (FANG) Well Characterized Challenge Material Working Group.
Matthew FriemanDepartment of Microbiology and Immunology, University of Maryland at Baltimore, Baltimore, Maryland, USA.
Jeffrey R KugelmanCenter for Genome Sciences, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA.
Jens H KuhnFilovirus Animal Nonclinical Group (FANG) Well Characterized Challenge Material Working Group Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland, USA.
Jules O'RearU.S. Food and Drug Administration, Silver Spring, Maryland, USA Filovirus Animal Nonclinical Group (FANG) Well Characterized Challenge Material Working Group.
Pardis C Sabeti
David E WentworthVirology, J. Craig Venter Institute, Rockville, Maryland, USA.
Michael R WileyCenter for Genome Sciences, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA.
Guo-Yun YuCenter for Genome Sciences, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA.
Threat Characterization Consortium
Shanmuga Sozhamannan
Christopher BradburneNational Security Systems Biology Center, Asymmetric Operations Sector, Johns Hopkins University, Applied Physics Laboratory, Laurel, Maryland, USA.
Gustavo PalaciosCenter for Genome Sciences, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA Filovirus Animal Nonclinical Group (FANG) Well Characterized Challenge Material Working Group jason.t.ladner.ctr@mail.mil gustavo.f.palacios.ctr@mail.mil.
United States Army Medical Research Institute of Infectious Diseases · USJohns Hopkins University Applied Physics Laboratory · USUniversity of Maryland, Baltimore · USCenter for Systems Biology · USDefense Threat Reduction Agency · USJ. Craig Venter Institute · USLos Alamos National Laboratory · USNational Institutes of Health · USUnited States Food and Drug Administration · US

Funding

NIAID NIH HHS HHSN272200700016I
6 · The paper itself

Abstract

Thanks to high-throughput sequencing technologies, genome sequencing has become a common component in nearly all aspects of viral research; thus, we are experiencing an explosion in both the number of available genome sequences and the number of institutions producing such data. However, there are currently no common standards used to convey the quality, and therefore utility, of these various genome sequences. Here, we propose five "standard" categories that encompass all stages of viral genome finishing, and we define them using simple criteria that are agnostic to the technology used for sequencing. We also provide genome finishing recommendations for various downstream applications, keeping in mind the cost-benefit trade-offs associated with different levels of finishing. Our goal is to define a common vocabulary that will allow comparison of genome quality across different research groups, sequencing platforms, and assembly techniques.

Indexed as

Genome, ViralHigh-Throughput Nucleotide SequencingViruses

Identifiers

PMID24939889
PMCPMC4068259
OpenAlexW2145946085

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.