ArticleAnalytical and bioanalytical chemistry2014
Evaluation of ultra high-performance [corrected] liquid chromatography columns for the analysis of unmodified and antisense oligonucleotides.
Article in Analytical and bioanalytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Development of Enhanced Separation Techniques for Oligonucleotides Utilizing Mixed-Mode Chromatography and 2D-LC/UV/MS Analysis.Journal of separation science · 2025Article
- Challenges and emerging trends in liquid chromatography-based analyses of mRNA pharmaceuticals.Journal of pharmaceutical and biomedical analysis · 2023Review
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- Oligonucleotides Isolation and Separation-A Review on Adsorbent Selection.International journal of molecular sciences · 2022Review
- Reconsidering the Chemical Nature of Strand Breaks Derived from Abasic Sites in Cellular DNA: Evidence for 3'-Glutathionylation.Journal of the American Chemical Society · 2022Article
- Unexpected Complexity in the Products Arising from NaOH-, Heat-, Amine-, and Glycosylase-Induced Strand Cleavage at an Abasic Site in DNA.Chemical research in toxicology · 2022Article
- Synthesis of DNA Duplexes Containing Site-Specific Interstrand Cross-Links via Sequential Reductive Amination Reactions Involving Diamine Linkers and Abasic Sites on Complementary Oligodeoxynucleotides.Chemical research in toxicology · 2021Article
- Interstrand Cross-Link Formation Involving Reaction of a Mispaired Cytosine Residue with an Abasic Site in Duplex DNA.Chemical research in toxicology · 2021Article
- Analytical and preparative separation of phosphorothioated oligonucleotides: columns and ion-pair reagents.Analytical and bioanalytical chemistry · 2020Article
- Oligonucleotide analysis by hydrophilic interaction liquid chromatography-mass spectrometry in the absence of ion-pair reagents.Journal of chromatography. A · 2019Article
- Investigation of factors influencing the separation of diastereomers of phosphorothioated oligonucleotides.Analytical and bioanalytical chemistry · 2019Article
- HPLC methods for purity evaluation of man-made single-stranded RNAs.Scientific reports · 2019Article
- Nucleic acid separations using superficially porous silica particles.Journal of chromatography. A · 2016Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ultra high-performance [corrected] liquid chromatography has been used for the separation and analysis of unmodified and modified antisense oligonucleotides. For this reason, we tested various columns of low particle sizes in our analysis of unmodified and phosphorothioate oligonucleotides. The influence of both the type and concentration of ion-pair reagent on the retention of the studied biomolecules was tested. The developed methods were used for separation of unmodified oligonucleotides and to determine antisense oligonucleotides in human serum samples. The results proved that octadecyl and phenyl columns are the most selective in the resolution of oligonucleotides which differ in the position of single nucleotides in the sequence. The phenyl column was selected and applied for the analysis of phosphorothioate oligonucleotides in serum samples. The calibration plots showed good linearity within the test concentration ranges. The intra-day CV of the calibration curve slopes was in the range of 1.6 to 4.2 %. The limits of detection (LODs) were in the range of 0.11-0.16 μg mL(-1), while the limit of quantification (LOQ) values were between 0.35 and 0.51 μg mL(-1).
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