Evidence map›Paper›PMID 24938428›Full record

ArticleScientific reports2014

Analysis of interactions between SNARE proteins using imaging ellipsometer coupled with microfluidic array.

Cai Qi, Hong Zhang, Li Liu, Renke Yang, Yinke Yang, Tengfei Kang, Wenxin Hao, Gang Jin, Taijiao Jiang

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

  • Erratum issued
    Yang, Yinke [corrected to Yang, Renke]
5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

Cai Qi1] Institute of Biophysics, Chinese Academy of Sciences, #15, Datun Rd., Beijing, 100101, China [2] Institute of Equipment Technology, Chinese Academy of Inspection and Quarantine, #3, Gaobeidian North Rd., Beijing, 100123, China [3].
Hong Zhang1] Institute of Biophysics, Chinese Academy of Sciences, #15, Datun Rd., Beijing, 100101, China [2].
Li LiuInstitute of Mechanics, Chinese Academy of Sciences, #15, Beisihuan West Rd., Beijing, 100190, China.
Renke Yang
Yinke YangXiang-Ya Medical School, Central South University, #932, Lushan South Rd., Changsha, Hunan, 410083, China.
Tengfei KangInstitute of Mechanics, Chinese Academy of Sciences, #15, Beisihuan West Rd., Beijing, 100190, China.
Wenxin HaoInstitute of Mechanics, Chinese Academy of Sciences, #15, Beisihuan West Rd., Beijing, 100190, China.
Gang JinInstitute of Mechanics, Chinese Academy of Sciences, #15, Beisihuan West Rd., Beijing, 100190, China.
Taijiao JiangInstitute of Biophysics, Chinese Academy of Sciences, #15, Datun Rd., Beijing, 100101, China.
Chinese Academy of Sciences · CNInstitute of Biophysics · CNInstitute of Mechanics · CNCentral South University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) proteins are small and abundant membrane-bound proteins, whose specific interactions mediate membrane fusion during cell fusion or cellular trafficking. In this study, we report the use of a label-free method, called imaging ellipsometer to analyze the interactions among three SNAREs, namely Sec22p, Ykt6p and Sso2p. The SNAREs were immobilized on the silicon wafer and then analyzed in a pairwise mode with microfluidic array, leading us to discover the interactions between Ykt6p and Sso2p, Sec22p and Sso2p. Moreover, by using the real-time function of the imaging ellipsometer, we were able to obtain their association constants (K(A)) of about 10(4) M(-1). We argue that the use of imaging ellipsometer coupled with microfluidic device will deepen our understanding of the molecular mechanisms underlying membrane fusion process.

Indexed as

AlgorithmsElectrophoresis, Polyacrylamide GelEscherichia coliKineticsMicrofluidic Analytical TechniquesProtein BindingProtein Interaction MappingQa-SNARE ProteinsRecombinant ProteinsRefractometryR-SNARE ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTime FactorsQa-SNARE ProteinsRecombinant ProteinsR-SNARE ProteinsSaccharomyces cerevisiae ProteinsSec22 protein, S cerevisiaeSSO2 protein, S cerevisiaeYKT6 protein, S cerevisiae

Identifiers

PMID24938428
PMCPMC4061542
OpenAlexW2124328463

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.