ArticleThe Plant cell2014
A Single-Pore Residue Renders the Arabidopsis Root Anion Channel SLAH2 Highly Nitrate Selective.
Article in The Plant cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 80 citations in OpenAlex.
- Bioorganic studies on the nyctinastic leaf-movement of plants.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2026Review
- Interacting MeZFP29 and MebZIPW regulates MeNRT2.2 from cassava responding to nitrate signaling.Plant cell reports · 2025Article
- Applying traditional and machine learning-based GWAS approaches for marker-trait identification in wheat.Frontiers in plant science · 2025Article
- Potassium extrusion by plant cells: evolution from an emergency valve to a driver of long-distance transport.The New phytologist · 2025Review
- Chloride transport and homeostasis in plants.Quantitative plant biology · 2025Review
- Gaining or cutting SLAC: the evolution of plant guard cell signalling pathways.The New phytologist · 2024Article
- Mechanistic insights into phosphoactivation of SLAC1 in guard cell signaling.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Calcium signaling in plant mineral nutrition: From uptake to transport.Plant communications · 2023Review
- Unlocking the potentials of nitrate transporters at improving plant nitrogen use efficiency.Frontiers in plant science · 2023Review
- Molecular response and evolution of plant anion transport systems to abiotic stress.Plant molecular biology · 2022Review
- Root Pulling Force Across Drought in Maize Reveals Genotype by Environment Interactions and Candidate Genes.Frontiers in plant science · 2022Article
- Cassava (Frontiers in plant science · 2022Article
- Structural and Functional Insights into the Role of Guard Cell Ion Channels in Abiotic Stress-Induced Stomatal Closure.Plants (Basel, Switzerland) · 2021Review
- Structure and activity of SLAC1 channels for stomatal signaling in leaves.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Identification and Expression Analysis ofInternational journal of molecular sciences · 2021Article
- Preference of Proteomonas sulcata anion channelrhodopsin for NOScientific reports · 2021Article
- Physiological and Transcriptomic Responses to Nitrogen Deficiency inFrontiers in plant science · 2021Article
- Potential Networks of Nitrogen-Phosphorus-Potassium Channels and Transporters in Arabidopsis Roots at a Single Cell Resolution.Frontiers in plant science · 2021Article
- Plant Cyclic Nucleotide-Gated Channels: New Insights on Their Functions and Regulation.Plant physiology · 2020Article
- Nitrate in 2020: Thirty Years from Transport to Signaling Networks.The Plant cell · 2020Review
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In contrast to animal cells, plants use nitrate as a major source of nitrogen. Following the uptake of nitrate, this major macronutrient is fed into the vasculature for long-distance transport. The Arabidopsis thaliana shoot expresses the anion channel SLOW ANION CHANNEL1 (SLAC1) and its homolog SLAC1 HOMOLOGOUS3 (SLAH3), which prefer nitrate as substrate but cannot exclude chloride ions. By contrast, we identified SLAH2 as a nitrate-specific channel that is impermeable for chloride. To understand the molecular basis for nitrate selection in the SLAH2 channel, SLAC1 and SLAH2 were modeled to the structure of HiTehA, a distantly related bacterial member. Structure-guided site-directed mutations converted SLAC1 into a SLAH2-like nitrate-specific anion channel and vice versa. Our findings indicate that two pore-occluding phenylalanines constrict the pore. The selectivity filter of SLAC/SLAH anion channels is determined by the polarity of pore-lining residues located on alpha helix 3. Changing the polar character of a single amino acid side chain (Ser-228) to a nonpolar residue turned the nitrate-selective SLAH2 into a chloride/nitrate-permeable anion channel. Thus, the molecular basis of the anion specificity of SLAC/SLAH anion channels seems to be determined by the presence and constellation of polar side chains that act in concert with the two pore-occluding phenylalanines.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.