ArticleMolecular & cellular proteomics : MCP2014
NeuCode labels for relative protein quantification.
Article in Molecular & cellular proteomics : MCP, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 113 citations in OpenAlex.
- Proteome Birthdating: A Single-Sample Approach for Measuring Global Turnover Dynamics and "Protein Age".Bio-protocol · 2025Article
- Implications of tissue specific STING protein flux and abundance on inflammation and the development of targeted therapeutics.PloS one · 2025Article
- A Tutorial Review of Labeling Methods in Mass Spectrometry-Based Quantitative Proteomics.ACS measurement science au · 2024Review
- Identification of Proteomic Biomarkers of Acetaminophen-Induced Hepatotoxicity Using Stable Isotope Labeling.Methods in molecular biology (Clifton, N.J.) · 2024Article
- 6-PlexAnalytical chemistry · 2023Article
- Omics and systems view of innate immune pathways.Proteomics · 2023Review
- Proteomic approaches to study ubiquitinomics.Biochimica et biophysica acta. Gene regulatory mechanisms · 2023Review
- High-Throughput Quantitative Glycomics Enabled by 12-plex Isobaric Multiplex Labeling Reagents for Carbonyl-Containing Compound (SUGAR) Tags.Journal of proteome research · 2023Article
- Chemical isotope labeling for quantitative proteomics.Mass spectrometry reviews · 2023Review
- Coisolation of Peptide Pairs for Peptide Identification and MS/MS-Based Quantification.Analytical chemistry · 2022Article
- Neutron encoded derivatization of endothelial cell lysates for quantitation of aldehyde metabolites using nESI-LC-HRMS.Analytica chimica acta · 2022Article
- Quantitative Mass Spectrometry-Based Proteomics: An Overview.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Defining the carrier proteome limit for single-cell proteomics.Nature methods · 2021Article
- Complexome Profiling-Exploring Mitochondrial Protein Complexes in Health and Disease.Frontiers in cell and developmental biology · 2021Review
- Subcellular proteomics.Nature reviews. Methods primers · 2021Article
- Emerging mass spectrometry-based proteomics methodologies for novel biomedical applications.Biochemical Society transactions · 2020Review
- Isobaric Matching between Runs and Novel PSM-Level Normalization in MaxQuant Strongly Improve Reporter Ion-Based Quantification.Journal of proteome research · 2020Article
- Article
- Analyzing Ribosome Remodeling in Health and Disease.Proteomics · 2020Review
- 21-plex DiLeu Isobaric Tags for High-Throughput Quantitative Proteomics.Analytical chemistry · 2020Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
Abstract
We describe a synthesis strategy for the preparation of lysine isotopologues that differ in mass by as little as 6 mDa. We demonstrate that incorporation of these molecules into the proteomes of actively growing cells does not affect cellular proliferation, and we discuss how to use the embedded mass signatures (neutron encoding (NeuCode)) for multiplexed proteome quantification by means of high-resolution mass spectrometry. NeuCode SILAC amalgamates the quantitative accuracy of SILAC with the multiplexing of isobaric tags and, in doing so, offers up new opportunities for biological investigation. We applied NeuCode SILAC to examine the relationship between transcript and protein levels in yeast cells responding to environmental stress. Finally, we monitored the time-resolved responses of five signaling mutants in a single 18-plex experiment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.