ArticleMolecules and cells2014
Monitoring the differentiation and migration patterns of neural cells derived from human embryonic stem cells using a microfluidic culture system.
Article in Molecules and cells, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- Clinical Efficacy of Stem Cell Therapy in Neurotraumatic and Neurodegenerative Conditions: A Comparative Review.Tissue engineering and regenerative medicine · 2025Review
- Fabrication of Porous Collagen Scaffolds Containing Embedded Channels with Collagen Membrane Linings.Micromachines · 2024Article
- Microfluidics for Neuronal Cell and Circuit Engineering.Chemical reviews · 2022Review
- MatriGridBioengineering (Basel, Switzerland) · 2022Review
- Neurons-on-a-Chip:Molecules and cells · 2022Review
- Mouse Neural Stem Cell Differentiation and Human Adipose Mesenchymal Stem Cell Transdifferentiation Into Neuron- and Oligodendrocyte-like Cells With Myelination Potential.Stem cell reviews and reports · 2022Article
- Novel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Out.Nanomaterials (Basel, Switzerland) · 2021Article
- 3D-Printed Soft Lithography for Complex Compartmentalized Microfluidic Neural Devices.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis.World journal of stem cells · 2019Review
- Multi-compartment Microfluidic Device Geometry and Covalently Bound Poly-D-Lysine Influence Neuronal Maturation.Frontiers in bioengineering and biotechnology · 2019Article
- Stem cells technology: a powerful tool behind new brain treatments.Drug delivery and translational research · 2018Review
- Retrograde interferon-gamma signaling induces major histocompatibility class I expression in human-induced pluripotent stem cell-derived neurons.Annals of clinical and translational neurology · 2018Article
- A Gal-MµS Device to Evaluate Cell Migratory Response to Combined Galvano-Chemotactic Fields.Biosensors · 2017Article
- Analytical Techniques in Neuroscience: Recent Advances in Imaging, Separation, and Electrochemical Methods.Analytical chemistry · 2017Article
- Microfluidic systems for stem cell-based neural tissue engineering.Lab on a chip · 2016Review
- Depletion of Inositol Polyphosphate 4-Phosphatase II Suppresses Callosal Axon Formation in the Developing Mice.Molecules and cells · 2016Article
- Semaphorin 3C Released from a Biocompatible Hydrogel Guides and Promotes Axonal Growth of Rodent and Human Dopaminergic Neurons.Tissue engineering. Part A · 2016Article
- Advances in microfluidic platforms for analyzing and regulating human pluripotent stem cells.Current opinion in genetics & development · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microfluidics can provide unique experimental tools to visualize the development of neural structures within a microscale device, which is followed by guidance of neurite growth in the axonal isolation compartment. We utilized microfluidics technology to monitor the differentiation and migration of neural cells derived from human embryonic stem cells (hESCs). We co-cultured hESCs with PA6 stromal cells, and isolated neural rosette-like structures, which subsequently formed neurospheres in suspension culture. Tuj1-positive neural cells, but not nestin-positive neural precursor cells (NPCs), were able to enter the microfluidics grooves (microchannels), suggesting that neural cell-migratory capacity was dependent upon neuronal differentiation stage. We also showed that bundles of axons formed and extended into the microchannels. Taken together, these results demonstrated that microfluidics technology can provide useful tools to study neurite outgrowth and axon guidance of neural cells, which are derived from human embryonic stem cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.