ArticlePloS one2014
Controlling osteogenic stem cell differentiation via soft bioinspired hydrogels.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed.
- Bone-Derived dECM Hydrogels Support Tunable Microenvironments for In Vitro Osteogenic Differentiation.Advanced healthcare materials · 2026Article
- Osteogenic Differentiation Properties of BLGFs/ODEX Hydrogel Doped With Collagen Derived FromInternational journal of biomaterials · 2026Article
- Recent Advances in Peptide-Functionalized Hydrogels for Bone Tissue Engineering.ACS biomaterials science & engineering · 2025Review
- Protease-degradable hydrogels with multifunctional biomimetic peptides for bone tissue engineering.Frontiers in bioengineering and biotechnology · 2023Article
- Crosslinker structure modulates bulk mechanical properties and dictates hMSC behavior on hyaluronic acid hydrogels.Acta biomaterialia · 2023Article
- Simulated Microgravity Remodels Extracellular Matrix of Osteocommitted Mesenchymal Stromal Cells.International journal of molecular sciences · 2021Article
- Bone Matrix Non-Collagenous Proteins in Tissue Engineering: Creating New Bone by Mimicking the Extracellular Matrix.Polymers · 2021Review
- Customized hydrogel substrates for serum-free expansion of functional hMSCs.Biomaterials science · 2020Article
- Synthesis and characterization of osteoinductive visible light-activated adhesive composites with antimicrobial properties.Journal of tissue engineering and regenerative medicine · 2020Article
- Tissue engineered bone mimetics to study bone disorders ex vivo: Role of bioinspired materials.Biomaterials · 2019Review
- Human Adipose-Derived Hydrogel Characterization Based onStem cells international · 2019Article
- Microenvironmental Regulation of Chondrocyte Plasticity in Endochondral Repair-A New Frontier for Developmental Engineering.Frontiers in bioengineering and biotechnology · 2018Review
- Can a Biodegradable Implanted Bilayered Drug Delivery System Loaded with BMP-2/BMP-12 Take an Effective Role in the Biological Repair Process of Bone-Tendon Injuries? A Preliminary Report.Journal of pharmaceutics · 2017Article
- A Review of Injectable Polymeric Hydrogel Systems for Application in Bone Tissue Engineering.Molecules (Basel, Switzerland) · 2016Review
- The In Vitro and In Vivo Response to MMP-Sensitive Poly(Ethylene Glycol) Hydrogels.Annals of biomedical engineering · 2016Article
- Hydrogel arrays formed via differential wettability patterning enable combinatorial screening of stem cell behavior.Acta biomaterialia · 2016Article
- Mechanical influence of tissue culture plates and extracellular matrix on mesenchymal stem cell behavior: A topical review.International journal of immunopathology and pharmacology · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Osteogenic differentiation of human mesenchymal stem cells (hMSCs) is guided by various physical and biochemical factors. Among these factors, modulus (i.e., rigidiy) of the ECM has gained significant attention as a physical osteoinductive signal that can contribute to endochondral ossification of a cartilaginous skeletal template. However, MSCs also participate in intramembranous bone formation, which occurs de novo from within or on a more compliant tissue environment. To further understand the role of the matrix interactions in this process, we evaluated osteogenic differentiation of hMSCs cultured on low moduli (102, 390 or 970 Pa) poly(N-isopropylacrylamide) (p(NIPAAm)) based semi-interpenetrating networks (sIPN) modified with the integrin engaging peptide bsp-RGD(15) (0, 105 or 210 µM). Cell adhesion, proliferation, and osteogenic differentiation of hMSCs, as measured by alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2), bone sialoprotein-2 (iBSP), and osteocalcien (OCN) protein expression, was highest on substrates with the highest modulus and peptide concentrations. However, within this range of substrate stiffness, many osteogenic cellular functions were enhanced by increasing either the modulus or the peptide density. These findings suggest that within a compliant and low modulus substrate, a high affinity adhesive ligand serves as a substitute for a rigid matrix to foster osteogenic differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.