ArticlePloS one2014
An unusual abscisic acid and gibberellic acid synergism increases somatic embryogenesis, facilitates its genetic analysis and improves transformation in Medicago truncatula.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 36 citations in OpenAlex.
- Molecular regulators of regeneration and strategies for overcoming genotype-dependent recalcitrance in wheat (Triticum aestivum).Communications biology · 2026Review
- The multifaceted role of gibberellins in somatic embryogenesis: fromFrontiers in plant science · 2026Review
- Identification of SUMO Proteins and Their Expression Profile During Induction of Somatic Embryogenesis inInternational journal of molecular sciences · 2025Article
- In Vitro Morphogenesis of Tobacco: Modulation of Endogenous Growth Regulators by Tulsi (Holy Basil).Plants (Basel, Switzerland) · 2024Article
- Changes and transcriptome regulation of endogenous hormones during somatic embryogenesis inFrontiers in plant science · 2023Article
- Article
- miRNAs as key regulators via targeting the phytohormone signaling pathways during somatic embryogenesis of plants.3 Biotech · 2020Review
- MtGA2ox10 encoding C20-GA2-oxidase regulates rhizobial infection and nodule development in Medicago truncatula.Scientific reports · 2019Article
- Hormone and RNA-seq analyses reveal the mechanisms underlying differences in seed vigour at different maize ear positions.Plant molecular biology · 2019Article
- Heat Stress in Legume Seed Setting: Effects, Causes, and Future Prospects.Frontiers in plant science · 2019Article
- Somatic Embryogenesis in theFrontiers in plant science · 2019Review
- Oil body biogenesis and biotechnology in legume seeds.Plant cell reports · 2017Review
- Comparative Transcriptome Analysis of SE initial dedifferentiation in cotton of different SE capability.Scientific reports · 2017Article
- Article
- Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula.Journal of visualized experiments : JoVE · 2015Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Somatic embryogenesis (SE) can be readily induced in leaf explants of the Jemalong 2HA genotype of the model legume Medicago truncatula by auxin and cytokinin, but rarely in wild-type Jemalong. Gibberellic acid (GA), a hormone not included in the medium, appears to act in Arabidopsis as a repressor of the embryonic state such that low ABA (abscisic acid): GA ratios will inhibit SE. It was important to evaluate the GA effect in M. truncatula in order to formulate generic SE mechanisms, given the Arabidopsis information. It was surprising to find that low ABA:GA ratios in M. truncatula acted synergistically to stimulate SE. The unusual synergism between GA and ABA in inducing SE has utility in improving SE for regeneration and transformation in M. truncatula. Expression of genes previously shown to be important in M. truncatula SE was not increased. In investigating genes previously studied in GA investigations of Arabidopsis SE, there was increased expression of GA2ox and decreased expression of PICKLE, a negative regulator of SE in Arabidopsis. We suggest that in M. truncatula there are different ABA:GA ratios required for down-regulating the PICKLE gene, a repressor of the embryonic state. In M. truncatula it is a low ABA:GA ratio while in Arabidopsis it is a high ABA:GA ratio. In different species the expression of key genes is probably related to differences in how the hormone networks optimise their expression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.