ArticlePloS one2014
Optimized cryopreservation of mixed microbial communities for conserved functionality and diversity.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed.
- Smartphone-based multispectral autofluorescence analysis of bacteria mixtures of staphylococci using convolutional neural network.Journal of biological engineering · 2026Article
- Type I Sourdough Preservation Strategies and the Contribution of Microbial Biological Resource Centers to Biodiversity Protection: A Narrative Review.Foods (Basel, Switzerland) · 2025Review
- Preserving the Biotransformation Potential of Activated Sludge in Time: Toward Reproducible Incubation Experiments for Persistence Assessment.Environmental science & technology · 2025Article
- Impact of Fecal Microbiota Transplant Formulations, Storage Conditions, and Duration on Bacterial Viability, Functionality, and Clinical Outcomes in Patients with RecurrentMicroorganisms · 2025Article
- Effects of Different Preservation Methods on the Structure and Diversity of Intestinal Microbiota of Marine Fishes.Current microbiology · 2025Article
- Evaluating long-term stool preservation methods for maximizing the recovery of viable human fecal microbiota.Gut microbes reports · 2025Article
- Plant miRNAs influence soil bacterial growth and amino acid uptake, restructuring community composition.ISME communications · 2025Article
- Investigation of Freezing and Freeze-Drying for Preserving and Re-Using a Whole Microbial Cheese Community.Foods (Basel, Switzerland) · 2024Article
- Impact of cryoprotective agents on human gut microbes and in vitro stabilized artificial gut microbiota communities.Microbial biotechnology · 2024Article
- A cryopreservation method to recover laboratory- and field-derived bacterial communities from mosquito larval habitats.PLoS neglected tropical diseases · 2023Article
- Review
- A Reproducible and Tunable Synthetic Soil Microbial Community Provides New Insights into Microbial Ecology.mSystems · 2022Article
- Optimization of Preservation Methods Provides Insights into Photosynthetic Picoeukaryotes in Lakes.Microbiology spectrum · 2022Article
- Effect of cryopreservation medium conditions on growth and isolation of gut anaerobes from human faecal samples.Microbiome · 2022Article
- Growth Performance, Biochemical Composition and Nutrient Recovery Ability of Twelve Microalgae Consortia Isolated from Various Local Organic Wastes Grown on Nano-Filtered Pig Slurry.Molecules (Basel, Switzerland) · 2022Article
- BIOME-Preserve: A novel storage and transport medium for preserving anaerobic microbiota samples for culture recovery.PloS one · 2022Article
- Storage of soil microbiome for application in sustainable agriculture: prospects and challenges.Environmental science and pollution research international · 2022Review
- Benchmarking DNA isolation kits used in analyses of the urinary microbiome.Scientific reports · 2021Article
- Prevalence of ESBL, AmpC and Carbapenemase-Producing Enterobacterales Isolated from Raw Vegetables Retailed in Romania.Foods (Basel, Switzerland) · 2020Article
- A Recent Overview of Microbes and Microbiome Preservation.Indian journal of microbiology · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of mixed microbial communities (microbiomes) for biotechnological applications has steadily increased over the past decades. However, these microbiomes are not readily available from public culture collections, hampering their potential for widespread use. The main reason for this lack of availability is the lack of an effective cryopreservation protocol. Due to this critical need, we evaluated the functionality as well as the community structure of three different types of microbiomes before and after cryopreservation with two cryoprotective agents (CPA). Microbiomes were selected based upon relevance towards applications: (1) a methanotrophic co-culture (MOB), with potential for mitigation of greenhouse gas emissions, environmental pollutants removal and bioplastics production; (2) an oxygen limited autotrophic nitrification/denitrification (OLAND) biofilm, with enhanced economic and ecological benefits for wastewater treatment, and (3) fecal material from a human donor, with potential applications for fecal transplants and pre/probiotics research. After three months of cryopreservation at -80 °C, we found that metabolic activity, in terms of the specific activity recovery of MOB, aerobic ammonium oxidizing bacteria (AerAOB) and anaerobic AOB (AnAOB, anammox) in the OLAND mixed culture, resumes sooner when one of our selected CPA [dimethyl sulfoxide (DMSO) and DMSO plus trehalose and tryptic soy broth (DMSO+TT)] was added. However, the activity of the fecal community was not influenced by the CPA addition, although the preservation of the community structure (as determined by 16S rRNA gene sequencing) was enhanced by addition of CPA. In summary, we have evaluated a cryopreservation protocol that succeeded in preserving both community structure and functionality of value-added microbiomes. This will allow individual laboratories and culture collections to boost the use of microbiomes in biotechnological applications.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.