Evidence map›Paper›PMID 24936866›Full record

ArticlePloS one2014

Nuclear NF-κB p65 in peripheral blood mononuclear cells correlates with urinary MCP-1, RANTES and the severity of type 2 diabetic nephropathy.

Bin Yi, Xiaofang Hu, Hao Zhang, Jing Huang, Jishi Liu, Jing Hu, Wei Li, Lihua Huang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed, 1 pooled it
1.8field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.

  1. Pooled it
  2. Article
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  5. Article
  6. Article
  7. Review
  8. Diabetic nephropathy patients show hyper-responsiveness to N6-carboxymethyllysine.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2022
    Observational
  9. Article
  10. Accelerated Kidney Aging in Diabetes Mellitus.Oxidative medicine and cellular longevity · 2020
    Review
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  12. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Bin YiDepartment of Nephrology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
Xiaofang HuDepartment of Nephrology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
Hao ZhangDepartment of Nephrology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
Jing HuangDepartment of Nephrology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
Jishi LiuDepartment of Nephrology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
Jing HuDepartment of Nephrology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
Wei LiDepartment of Nephrology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
Lihua HuangMedical experimental center, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
Third Xiangya Hospital · CNCentral South University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimsTo investigate if nuclear NF-κB p65 expression in ex vivo isolated peripheral blood mononuclear cells correlates with urinary MCP-1 or RANTES and the severity of type 2 diabetic nephropathy.

methodsAccording to their urinary albumin-to-creatinine ratio (uACR), 107 patients with type 2 diabetes (eGFR >60 ml/min) were divided into normal albuminuria group (DN0 group, 38 cases), microalbuminuria group (DN1 group, 38 cases), and macroalbuminuria group (DN2 group, 31 cases), compared with matched healthy normal control group (NC group, 30 cases). Nuclear NF-κB p65 protein expression levels in peripheral blood mononuclear cells were detected by western blotting. Real-time quantitative polymerase chain reaction was used to detect NF-κB p65 mRNA expression and ELISA assay was used to detect the levels of urinary MCP-1 and RANTES.

resultsNuclear NF-κB p65 protein and NF-κB p65 mRNA expression levels in peripheral blood mononuclear cells, urinary MCP-1/Cr and RANTES/Cr were all significantly higher in all diabetes groups as compared with NC group. In particular, the increase of nuclear NF-κB p65 protein and NF-κB p65 mRNA expressions, urinary MCP-1/Cr and RANTES/Cr all correlated with the severity of type 2 diabetic nephropathy as indicated by the increase in uACR. Pearson correlation analysis indicated that both urinary MCP-1/Cr and RANTES/Cr were positively correlated with nuclear NF-κB p65 protein or NF-κB p65 mRNA levels. Stepwise multiple regression analysis showed that nuclear NF-κB p65 protein or NF-κB p65 mRNA was an independent variable for urinary MCP-1/Cr, and MCP-1/Cr and RANTES/Cr were two independent variables for uACR.

conclusionOur research demonstrates that nuclear NF-κB p65 protein and mRNA expressions in ex vivo isolated peripheral blood mononuclear cells well correlate with urinary MCP-1/Cr, RANTES/Cr and the severity of type 2 diabetic nephropathy.

Indexed as

AgedBiomarkersCase-Control StudiesCell NucleusChemokine CCL2Chemokine CCL5Diabetes Mellitus, Type 2Diabetic NephropathiesFemaleGene ExpressionGlomerular Filtration RateHumansLeukocytes, MononuclearMaleMiddle AgedSeverity of Illness IndexBiomarkersCCL2 protein, humanCCL5 protein, humanChemokine CCL2Chemokine CCL5RELA protein, humanTranscription Factor RelA

Identifiers

PMID24936866
PMCPMC4061032
OpenAlexW2077825206

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.