ArticleScientific reports2014
Efficient genetic manipulation of the NOD-Rag1-/-IL2RgammaC-null mouse by combining in vitro fertilization and CRISPR/Cas9 technology.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- A streamlined approach for gene editing in non-obese diabetes (NOD) mice via CRISPR/Cas9.Laboratory animal research · 2026Article
- Humanized mouse models in MDS.Cell death & disease · 2025Review
- The IsletTester Mouse: An Immunodeficient Model With Stable Hyperglycemia for the Study of Human Islets.Diabetes · 2025Article
- Modeling HBV Infection and Therapy in Immunodeficient NOD-Rag1-/-IL2RgammaC-null (NRG) Fumarylacetoacetate Hydrolase (FAH) Knockout Mice with Human Chimeric Liver.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Genic constraint against nonsynonymous variation across the mouse genome.BMC genomics · 2023Article
- Review
- Patch grafting of organoids of stem/progenitors into solid organs can correct genetic-based disease states.Biomaterials · 2022Article
- Liver-humanized mice: A translational strategy to study metabolic disorders.Journal of cellular physiology · 2022Review
- BAHCC1 binds H3K27me3 via a conserved BAH module to mediate gene silencing and oncogenesis.Nature genetics · 2020Article
- Designing a therapeutic hepatitis B vaccine to circumvent immune tolerance.Human vaccines & immunotherapeutics · 2020Article
- Two base pair deletion in IL2 receptor γ gene in NOD/SCID mice induces a highly severe immunodeficiency.Laboratory animal research · 2020Article
- Genome editing methods in animal models.Animal cells and systems · 2020Review
- Using CRISPR/Cas9 to model human liver disease.JHEP reports : innovation in hepatology · 2019Review
- Generating mouse models for biomedical research: technological advances.Disease models & mechanisms · 2019Review
- SENP3-mediated host defense response contains HBV replication and restores protein synthesis.PloS one · 2019Article
- [Generation of a new strain of NOD/SCID/IL2RγNan fang yi ke da xue xue bao = Journal of Southern Medical University · 2018Article
- Comparison of Various Nuclear Localization Signal-Fused Cas9 Proteins andG3 (Bethesda, Md.) · 2018Article
- Bacterial andFrontiers in immunology · 2018Article
- Rapid generation of Col7a1Laboratory investigation; a journal of technical methods and pathology · 2017Article
- HTLV-1 Infection and Neuropathogenesis in the Context of Rag1Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Humanized mouse models have become increasingly important and widely used in modeling human diseases in biomedical research. Immunodeficient mice such as NOD-Rag1-/-IL2RgammaC-null (NRG) or NOD-SCID-IL2RgammaC-null (NSG) mice are critical for efficient engraftment of human cells or tissues. However, their genetic modification remains challenging due to a lack of embryonic stem cells and difficulty in the collection of timed embryos after superovulation. Here, we report the generation of gene knockout NRG mice by combining in vitro fertilization (IVF) and CRISPR/Cas9 technology. Sufficient numbers of fertilized embryos were produced through IVF, and a high rate of Fah gene targeting was achieved with microinjection of Cas9 mRNA, gRNA and single strand oligonucleotide DNA (ssDNA) into the embryos. The technology paves the way to construct NRG or NSG mutant mice to facilitate new humanized mouse models. The technology can also be readily adapted to introduce mutations in other species such as swine and non-human primates.
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