ArticleScientific reports2014
Multi-wavelength photoacoustic imaging of inducible tyrosinase reporter gene expression in xenograft tumors.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 80 citations in OpenAlex.
- Performance characterization of light-emitting diode array-based photoacoustic computed tomography (LED-PACT).Biomedical optics express · 2025Article
- Controlling the sound of light: photoswitching optoacoustic imaging.Nature methods · 2024Review
- Hybrid confocal fluorescence and photoacoustic microscopy for the label-free investigation of melanin accumulation in fish scales.Scientific reports · 2022Article
- Melanins as Sustainable Resources for Advanced Biotechnological Applications.Global challenges (Hoboken, NJ) · 2021Review
- Enhanced Antitumoral Activity and Photoacoustic Imaging Properties of AuNP-Enriched Endothelial Colony Forming Cells on Melanoma.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Photoacoustic Tomography Opening New Paradigms in Biomedical Imaging.Advances in experimental medicine and biology · 2021Article
- Photoacoustic Neuroimaging - Perspectives on a Maturing Imaging Technique and its Applications in Neuroscience.Frontiers in neuroscience · 2021Review
- Towards non-contact photoacoustic imaging [review].Photoacoustics · 2020Review
- Wide-field polygon-scanning photoacoustic microscopy of oxygen saturation at 1-MHz A-line rate.Photoacoustics · 2020Article
- Fluence-matching technique using photoacoustic radiofrequency spectra for improving estimates of oxygen saturation.Photoacoustics · 2020Article
- Photoacoustic imaging of kidney fibrosis for assessing pretransplant organ quality.JCI insight · 2020Article
- Sensitivity Enhanced Photoacoustic Imaging Using a High-Frequency PZT Transducer with an Integrated Front-End Amplifier.Sensors (Basel, Switzerland) · 2020Article
- Complete-noncontact photoacoustic microscopy by detection of initial pressures using a 3×3 coupler-based fiber-optic interferometer.Biomedical optics express · 2020Article
- Estimating blood oxygenation from photoacoustic images: can a simple linear spectroscopic inversion ever work?Journal of biomedical optics · 2019Article
- Development of a Human Photoacoustic Imaging Reporter Gene Using the Clinical Dye Indocyanine Green.Radiology. Imaging cancer · 2019Article
- Optical-resolution photoacoustic microscopy of oxygen saturation with nonlinear compensation.Biomedical optics express · 2019Article
- Photoacoustic imaging of clofazimine hydrochloride nanoparticle accumulation in cancerous vs normal prostates.PloS one · 2019Article
- Transient Triplet Differential (TTD) Method for Background Free Photoacoustic Imaging.Scientific reports · 2018Article
- Tyrosinase-Based Reporter Gene for Photoacoustic Imaging of MicroRNA-9 Regulated by DNA Methylation in Living Subjects.Molecular therapy. Nucleic acids · 2018Article
- Detecting human melanoma cell re-differentiation following BRAF or heat shock protein 90 inhibition using photoacoustic and magnetic resonance imaging.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Photoacoustic imaging is an emerging hybrid imaging technology capable of breaking through resolution limits of pure optical imaging technologies imposed by optical-scattering to provide fine-resolution optical contrast information in deep tissues. We demonstrate the ability of multi-wavelength photoacoustic imaging to estimate relative gene expression distributions using an inducible expression system and co-register images with hemoglobin oxygen saturation estimates and micro-ultrasound data. Tyrosinase, the rate-limiting enzyme in melanin production, is used as a reporter gene owing to its strong optical absorption and enzymatic amplification mechanism. Tetracycline-inducible melanin expression is turned on via doxycycline treatment in vivo. Serial multi-wavelength imaging reveals very low estimated melanin expression in tumors prior to doxycycline treatment or in tumors with no tyrosinase gene present, but strong signals after melanin induction in tumors tagged with the tyrosinase reporter. The combination of new inducible reporters and high-resolution photoacoustic and micro-ultrasound technology is poised to bring a new dimension to the study of gene expression in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.