ArticleStem cell reports2014
Heterochromatin dynamics during the differentiation process revealed by the DNA methylation reporter mouse, MethylRO.
Article in Stem cell reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 49 citations in OpenAlex.
- Recent advances in methodologies of epigenomics.Epigenomics · 2026Review
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- Visualizing histone H4K20me1 in knock-in mice expressing the mCherry-tagged modification-specific intracellular antibody.Histochemistry and cell biology · 2024Article
- Toward the Development of Epigenome Editing-Based Therapeutics: Potentials and Challenges.International journal of molecular sciences · 2023Review
- Targeted DNA Methylation in Mouse Early Embryos.Methods in molecular biology (Clifton, N.J.) · 2023Article
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- Development and application of novel BiFC probes for cell sorting based on epigenetic modification.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2022Article
- Live-cell imaging probes to track chromatin modification dynamics.Microscopy (Oxford, England) · 2021Article
- Antifibrotic effect of lung-resident progenitor cells with high aldehyde dehydrogenase activity.Stem cell research & therapy · 2021Article
- Symmetrically dimethylated histone H3R2 promotes global transcription during minor zygotic genome activation in mouse pronuclei.Scientific reports · 2021Article
- Bioengineering in vitro models of embryonic development.Stem cell reports · 2021Review
- Liver Organoids: Recent Developments, Limitations and Potential.Frontiers in medicine · 2021Review
- 3D convolutional neural networks-based segmentation to acquire quantitative criteria of the nucleus during mouse embryogenesis.NPJ systems biology and applications · 2020Article
- Editing DNA Methylation in Mammalian Embryos.International journal of molecular sciences · 2020Review
- The Promises and Challenges of Toxico-Epigenomics: Environmental Chemicals and Their Impacts on the Epigenome.Environmental health perspectives · 2020Article
- Tetraploidy causes chromosomal instability in acentriolar mouse embryos.Nature communications · 2019Article
- Analysis of the 4q35 chromatin organization reveals distinct long-range interactions in patients affected with Facio-Scapulo-Humeral Dystrophy.Scientific reports · 2019Article
- Nuclear formation induced by DNA-conjugated beads in living fertilised mouse egg.Scientific reports · 2019Article
- A microfluidic device for isolating intact chromosomes from single mammalian cells and probing their folding stability by controlling solution conditions.Scientific reports · 2018Article
- AAV2-Mediated Transduction of the Mouse Retina After Optic Nerve Injury.Investigative ophthalmology & visual science · 2017Article
Corrections and comments
- Erratum issued
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In mammals, DNA is methylated at CpG sites, which play pivotal roles in gene silencing and chromatin organization. Furthermore, DNA methylation undergoes dynamic changes during development, differentiation, and in pathological processes. The conventional methods represent snapshots; therefore, the dynamics of this marker within living organisms remains unclear. To track this dynamics, we made a knockin mouse that expresses a red fluorescent protein (RFP)-fused methyl-CpG-binding domain (MBD) protein from the ROSA26 locus ubiquitously; we named it MethylRO (methylation probe in ROSA26 locus). Using this mouse, we performed RFP-mediated methylated DNA immunoprecipitation sequencing (MeDIP-seq), whole-body section analysis, and live-cell imaging. We discovered that mobility and pattern of heterochromatin as well as DNA methylation signal intensity inside the nuclei can be markers for cellular differentiation status. Thus, the MethylRO mouse represents a powerful bioresource and technique for DNA methylation dynamics studies in developmental biology, stem cell biology, as well as in disease states.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.