ArticleStem cell reports2014
Single-cell gene expression profiles define self-renewing, pluripotent, and lineage primed states of human pluripotent stem cells.
Article in Stem cell reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed, 88 citations in OpenAlex.
- Article
- Pluripotent cell states and fates in human embryo models.Development (Cambridge, England) · 2025Review
- Three-dimensional stem cell models of mammalian gastrulation.BioEssays : news and reviews in molecular, cellular and developmental biology · 2024Review
- Article
- Connexin 25 maintains self-renewal and functions of airway basal cells for airway regeneration.Stem cell research & therapy · 2024Article
- Individual variation in the emergence of anterior-to-posterior neural fates from human pluripotent stem cells.Stem cell reports · 2024Article
- Complex regulatory networks influence pluripotent cell state transitions in human iPSCs.Nature communications · 2024Article
- KDM3A and KDM3B Maintain Naïve Pluripotency Through the Regulation of Alternative Splicing.bioRxiv : the preprint server for biology · 2024Article
- Laminin111-based defined culture promoting self-renewing human pluripotent stem cells with properties of the early post-implantation epiblast.Stem cell reports · 2022Article
- Cell type determination for cardiac differentiation occurs soon after seeding of human-induced pluripotent stem cells.Genome biology · 2022Article
- Single cell heterogeneity in human pluripotent stem cells.BMB reports · 2021Article
- Capture of Mouse and Human Stem Cells with Features of Formative Pluripotency.Cell stem cell · 2021Article
- Mapping germ-layer specification preventing genes in hPSCs via genome-scale CRISPR screening.iScience · 2021Article
- Generation and trapping of a mesoderm biased state of human pluripotency.Nature communications · 2020Article
- Individual Control and Quantification of 3D Spheroids in a High-Density Microfluidic Droplet Array.Cell reports · 2020Article
- Mapping the structure and biological functions within mesenchymal bodies using microfluidics.Science advances · 2020Article
- The application of cell surface markers to demarcate distinct human pluripotent states.Experimental cell research · 2020Review
- A Darwinian and Physical Look at Stem Cell Biology Helps Understanding the Role of Stochasticity in Development.Frontiers in cell and developmental biology · 2020Article
- FGF9 induces functional differentiation to Schwann cells from human adipose derived stem cells.Theranostics · 2020Article
- Capacitation of human naïve pluripotent stem cells for multi-lineage differentiation.Development (Cambridge, England) · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pluripotent stem cells display significant heterogeneity in gene expression, but whether this diversity is an inherent feature of the pluripotent state remains unknown. Single-cell gene expression analysis in cell subsets defined by surface antigen expression revealed that human embryonic stem cell cultures exist as a continuum of cell states, even under defined conditions that drive self-renewal. The majority of the population expressed canonical pluripotency transcription factors and could differentiate into derivatives of all three germ layers. A minority subpopulation of cells displayed high self-renewal capacity, consistently high transcripts for all pluripotency-related genes studied, and no lineage priming. This subpopulation was characterized by its expression of a particular set of intercellular signaling molecules whose genes shared common regulatory features. Our data support a model of an inherently metastable self-renewing population that gives rise to a continuum of intermediate pluripotent states, which ultimately become primed for lineage specification.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.