Evidence map›Paper›PMID 24936470›Full record

ArticleStem cell reports2014

Human intestinal tissue with adult stem cell properties derived from pluripotent stem cells.

Ryan Forster, Kunitoshi Chiba, Lorian Schaeffer, Samuel G Regalado, Christine S Lai, Qing Gao, Samira Kiani, Henner F Farin, Hans Clevers, Gregory J Cost and 7 more

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 49 papers.

0numbers the graph read from it
0cells of the map it votes in
49citing papers in PubMed
7.2field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

49 citing papers in PubMed, 88 citations in OpenAlex.

  1. Article
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  10. Organoids as a new model system to study neural tube defects.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021
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  20. Towards Multi-Organoid Systems for Drug Screening Applications.Bioengineering (Basel, Switzerland) · 2018
    Review
4 · The record

Corrections and comments

  • Erratum issued
5 · Who and what money

Authors and funding

17 authors at 6 institutions in 3 countries.

Ryan ForsterDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720-3370, USA.
Kunitoshi ChibaDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720-3370, USA.
Lorian SchaefferDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720-3370, USA.
Samuel G RegaladoDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720-3370, USA.
Christine S LaiThe Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
Qing GaoThe Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
Samira KianiThe Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
Henner F FarinHubrecht Institute, Royal Netherlands Academy of Arts and Sciences, University Medical Center Utrecht, 3584 Utrecht, the Netherlands.
Hans CleversHubrecht Institute, Royal Netherlands Academy of Arts and Sciences, University Medical Center Utrecht, 3584 Utrecht, the Netherlands ; Skolkovo Institute of Science and Technology (Skoltech), Novaya str. 100, Skolkovo, Moscow Region 143025, Russia.
Gregory J CostSangamo BioSciences, Richmond, CA 94804, USA.
Andy ChanSangamo BioSciences, Richmond, CA 94804, USA.
Edward J RebarSangamo BioSciences, Richmond, CA 94804, USA.
Fyodor D UrnovSangamo BioSciences, Richmond, CA 94804, USA.
Philip D GregorySangamo BioSciences, Richmond, CA 94804, USA.
Lior PachterDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720-3370, USA ; Department of Mathematics and Computer Science, University of California, Berkeley, Berkeley, CA 94720-3840, USA.
Rudolf JaenischThe Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA ; Skolkovo Institute of Science and Technology (Skoltech), Novaya str. 100, Skolkovo, Moscow Region 143025, Russia ; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Dirk HockemeyerDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720-3370, USA.
University of California, Berkeley · USSangamo BioSciences (United States) · USWhitehead Institute for Biomedical Research · USMassachusetts Institute of Technology · USRoyal Netherlands Academy of Arts and Sciences · NLUniversity Medical Center Utrecht · NL

Funding

VIRUS PRODUCTION COREP30CA014051 · NCI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI MATTHEW G. VANDER HEIDEN · 1985 to 2026
$93.9M
THE MOLECULAR BASIS OF CELL FUNCTIONT32GM007232 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI BILDER, DAVID, UNAL, ELCIN · 1985 to 2022
$36.4M
GENOMIC IMPRINTING AND THE CLONING OF MICER37CA084198 · NCI · WHITEHEAD INSTITUTE FOR BIOMEDICAL RES · PI JAENISCH, RUDOLF · 2000 to 2008
$9.2M
Epigenetics, Stem Cells, and CancerR01CA087869 · NCI · WHITEHEAD INSTITUTE FOR BIOMEDICAL RES · PI JAENISCH, RUDOLF · 2001 to 2010
$8.2M
Univ of Calif Berkeley Chemistry-Biology Interface ProgramT32GM066698 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI CHANG, CHRISTOPHER J., FRANCIS, MATTHEW B · 2004 to 2023
$7.5M
Nuclear Cloning and the Reprogramming of the GenomeR01HD045022 · NICHD · WHITEHEAD INSTITUTE FOR BIOMEDICAL RES · PI JAENISCH, RUDOLF · 2003 to 2008
$4.7M
NCI NIH HHS P30 CA014051NCI NIH HHS R01 CA087869NCI NIH HHS R01-CA087869NCI NIH HHS R37 CA084198NCI NIH HHS R37-CA084198NICHD NIH HHS R01 HD045022NICHD NIH HHS R01-HD045022NIGMS NIH HHS 2T32GM007232-36NIGMS NIH HHS T32 GM007232NIGMS NIH HHS T32 GM066698
6 · The paper itself

Abstract

Genetically engineered human pluripotent stem cells (hPSCs) have been proposed as a source for transplantation therapies and are rapidly becoming valuable tools for human disease modeling. However, many applications are limited due to the lack of robust differentiation paradigms that allow for the isolation of defined functional tissues. Here, using an endogenous LGR5-GFP reporter, we derived adult stem cells from hPSCs that gave rise to functional human intestinal tissue comprising all major cell types of the intestine. Histological and functional analyses revealed that such human organoid cultures could be derived with high purity and with a composition and morphology similar to those of cultures obtained from human biopsies. Importantly, hPSC-derived organoids responded to the canonical signaling pathways that control self-renewal and differentiation in the adult human intestinal stem cell compartment. This adult stem cell system provides a platform for studying human intestinal disease in vitro using genetically engineered hPSCs.

Indexed as

Adult Stem CellsCells, CulturedGene Expression ProfilingHumansIntestinesPluripotent Stem CellsReceptors, G-Protein-CoupledLGR5 protein, humanReceptors, G-Protein-Coupled

Identifiers

PMID24936470
PMCPMC4050346
OpenAlexW2024681578

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.