ArticleStem cell reports2014
3D In vitro model of a functional epidermal permeability barrier from human embryonic stem cells and induced pluripotent stem cells.
Article in Stem cell reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
50 citing papers in PubMed.
- Article
- Bioengineering strategies for regeneration of skin integrity: A literature review.Regenerative therapy · 2025Review
- Cytosine Deaminase Base Editing to RestoreJID innovations : skin science from molecules to population health · 2023Article
- Interactions betweenInternational journal of molecular sciences · 2023Article
- Differentiation of Human Induced Pluripotent Stem Cells into Keratinocytes.Current protocols · 2022Article
- Skin-on-a-Chip Technology: Microengineering Physiologically Relevant In Vitro Skin Models.Pharmaceutics · 2022Review
- Epidermal Basement Membrane Substitutes for Bioengineering of Human Epidermal Equivalents.JID innovations : skin science from molecules to population health · 2022Article
- mRNA-Based Reprogramming Under Xeno-Free and Feeder-Free Conditions.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Directed differentiation of human pluripotent stem cells into epidermal stem and progenitor cells.Molecular biology reports · 2021Article
- Cellular human tissue-engineered skin substitutes investigated for deep and difficult to heal injuries.NPJ Regenerative medicine · 2021Review
- Inherited DNA Repair Defects Disrupt the Structure and Function of Human Skin.Cell stem cell · 2021Article
- hiPSC-Derived Epidermal Keratinocytes from Ichthyosis Patients Show Altered Expression of Cornification Markers.International journal of molecular sciences · 2021Article
- Rare Genetic Disorders: Novel Treatment Strategies and Insights Into Human Biology.Frontiers in genetics · 2021Article
- Retinoic acid receptor γ activation promotes differentiation of human induced pluripotent stem cells into esophageal epithelium.Journal of gastroenterology · 2020Article
- Multi-lineage Human iPSC-Derived Platforms for Disease Modeling and Drug Discovery.Cell stem cell · 2020Review
- Genome-Wide DNA Methylation Enhances Stemness in the Mechanical Selection of Tumor-Repopulating Cells.Frontiers in bioengineering and biotechnology · 2020Article
- Stagewise keratinocyte differentiation from human embryonic stem cells by defined signal transduction modulators.International journal of biological sciences · 2020Article
- Skin-on-a-Chip Device for Ex Vivo Monitoring of Transdermal Delivery of Drugs-Design, Fabrication, and Testing.Pharmaceutics · 2019Article
- Review
- Comparison of human isogeneic Wharton's jelly MSCs and iPSC-derived MSCs reveals differentiation-dependent metabolic responses to IFNG stimulation.Cell death & disease · 2019Article
Corrections and comments
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Authors and funding
17 authors.
Funding
Abstract
Cornification and epidermal barrier defects are associated with a number of clinically diverse skin disorders. However, a suitable in vitro model for studying normal barrier function and barrier defects is still lacking. Here, we demonstrate the generation of human epidermal equivalents (HEEs) from human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). HEEs are structurally similar to native epidermis, with a functional permeability barrier. We exposed a pure population of hESC/iPSC-derived keratinocytes, whose transcriptome corresponds to the gene signature of normal primary human keratinocytes (NHKs), to a sequential high-to-low humidity environment in an air/liquid interface culture. The resulting HEEs had all of the cellular strata of the human epidermis, with skin barrier properties similar to those of normal skin. Such HEEs generated from disease-specific iPSCs will be an invaluable tool not only for dissecting molecular mechanisms that lead to epidermal barrier defects but also for drug development and screening.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.