Evidence map›Paper›PMID 24936446›Full record

ArticleStem cell reports2014

Defining differences among perivascular cells derived from human pluripotent stem cells.

Maureen Wanjare, Sravanti Kusuma, Sharon Gerecht

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed.

  1. Review
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  3. Review
  4. Review
  5. Article
  6. Article
  7. Sourcing cells forFrontiers in medical technology · 2022
    Review
  8. Review
  9. Review
  10. Review
  11. Article
  12. iScience · 2021
    Article
  13. Article
  14. Article
  15. Review
  16. Article
  17. Article
  18. Article
  19. Article
  20. Lymphatic Tissue Engineering and Regeneration.Journal of biological engineering · 2018
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

3 authors.

Maureen WanjareDepartment of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center and Institute for NanoBioTechnology, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USA.
Sravanti KusumaDepartment of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center and Institute for NanoBioTechnology, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USA ; Department of Biomedical Engineering, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USA.
Sharon GerechtDepartment of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center and Institute for NanoBioTechnology, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USA ; Department of Materials Science and Engineering, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USA.

Funding

Engineered Stem Cell Microenvironments for controlled VasculogenesisR01HL107938 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI GERECHT, SHARON · 2011 to 2015
$2.1M
Directed Derivation of Endothelial Cells from Human Pluripotent Stem CellsF31HL112644 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI KUSUMA, SRAVANTI · 2012 to 2013
$84k
NHLBI NIH HHS F31 HL112644NHLBI NIH HHS F31HL112644NHLBI NIH HHS R01 HL107938NHLBI NIH HHS R01HL107938
6 · The paper itself

Abstract

Distinguishing between perivascular cell types remains a hurdle in vascular biology due to overlapping marker expressions and similar functionalities. Clarifying and defining heterogeneities in vitro among perivascular cells could lead to improved cell-based tissue regeneration strategies and a better understanding of human developmental processes. We studied contractile vascular smooth muscle cells (vSMCs), synthetic vSMCs, and pericytes derived from a common human pluripotent stem cell source. Using in vitro cultures, we show unique cell morphology, subcellular organelle organization (namely endoplasmic reticulum, mitochondria, and stress fibers), and expression of smooth muscle myosin heavy chain and elastin for each cell type. While differences in extracellular matrix deposition and remodeling were less pronounced, the multipotency, in vivo, migratory, invasion, and contractile functionalities are distinctive for each cell type. Overall, we define a repertoire of functional phenotypes in vitro specific for each of the human perivascular cell types, enabling their study and use in basic and translational research.

Indexed as

Cell DifferentiationElastinExtracellular Matrix ProteinsHumansMetalloendopeptidasesMuscle ContractionMuscle, Smooth, VascularMyocytes, Smooth MuscleMyosin Heavy ChainsPericytesPluripotent Stem CellsElastinExtracellular Matrix ProteinsMetalloendopeptidasesMyosin Heavy Chains

Identifiers

PMID24936446
PMCPMC4050491

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.