ArticleJournal of bacteriology2014
Brucella abortus depends on pyruvate phosphate dikinase and malic enzyme but not on Fbp and GlpX fructose-1,6-bisphosphatases for full virulence in laboratory models.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Ability ofMicroorganisms · 2026Article
- Genome-wide identification of metabolic and regulatory determinants of intracellular growth inbioRxiv : the preprint server for biology · 2026Article
- Disruption of the Nitric Oxide Reductase Operon viaMicroorganisms · 2025Article
- Identification of Brucella RS15060 as a novel type IV secretion system effector associated with bacterial virulence.Veterinary research · 2024Article
- Disruption of Erythritol Catabolism via the Deletion of Fructose-Bisphosphate Aldolase (Fba) and Transaldolase (Tal) as a Strategy to Improve theInternational journal of molecular sciences · 2024Article
- Vaccine-Elicited Antibodies Restrict Glucose Availability to Control Brucella Infection.The Journal of infectious diseases · 2024Article
- Genome-wide analysis of Brucella melitensis growth in spleen of infected mice allows rational selection of new vaccine candidates.PLoS pathogens · 2024Article
- A novel gluconeogenic route enables efficient use of erythritol in zoonoticFrontiers in veterinary science · 2024Article
- Brucella effectors NyxA and NyxB target SENP3 to modulate the subcellular localisation of nucleolar proteins.Nature communications · 2023Article
- Genome-wide analysis of Brucella melitensis genes required throughout intranasal infection in mice.PLoS pathogens · 2022Article
- Article
- MyD88-Dependent Glucose Restriction and Itaconate Production Control Brucella Infection.Infection and immunity · 2021Article
- Aconitate decarboxylase 1 participates in the control of pulmonary Brucella infection in mice.PLoS pathogens · 2021Article
- Uncovering the Hidden Credentials ofMicrobiology and molecular biology reviews : MMBR · 2021Review
- Article
- Convergent evolution of zoonoticProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Disruption of pyruvate phosphate dikinase in Brucella ovis PA COVeterinary research · 2020Article
- Development of attenuated live vaccine candidates against swine brucellosis in a non-zoonotic B. suis biovar 2 background.Veterinary research · 2020Article
- Article
- Glucose Oxidation to Pyruvate Is Not Essential forFrontiers in microbiology · 2020Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The brucellae are the etiological agents of brucellosis, a worldwide-distributed zoonosis. These bacteria are facultative intracellular parasites and thus are able to adjust their metabolism to the extra- and intracellular environments encountered during an infectious cycle. However, this aspect of Brucella biology is imperfectly understood, and the nutrients available in the intracellular niche are unknown. Here, we investigated the central pathways of C metabolism used by Brucella abortus by deleting the putative fructose-1,6-bisphosphatase (fbp and glpX), phosphoenolpyruvate carboxykinase (pckA), pyruvate phosphate dikinase (ppdK), and malic enzyme (mae) genes. In gluconeogenic but not in rich media, growth of ΔppdK and Δmae mutants was severely impaired and growth of the double Δfbp-ΔglpX mutant was reduced. In macrophages, only the ΔppdK and Δmae mutants showed reduced multiplication, and studies with the ΔppdK mutant confirmed that it reached the replicative niche. Similarly, only the ΔppdK and Δmae mutants were attenuated in mice, the former being cleared by week 10 and the latter persisting longer than 12 weeks. We also investigated the glyoxylate cycle. Although aceA (isocitrate lyase) promoter activity was enhanced in rich medium, aceA disruption had no effect in vitro or on multiplication in macrophages or mouse spleens. The results suggest that B. abortus grows intracellularly using a limited supply of 6-C (and 5-C) sugars that is compensated by glutamate and possibly other amino acids entering the Krebs cycle without a critical role of the glyoxylate shunt.
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