ArticleInfection and immunity2014
Elongation factor P and modifying enzyme PoxA are necessary for virulence of Shigella flexneri.
Article in Infection and immunity, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- Lithocholic acid induces T3SS-dependent formation of invasion-competentInfection and immunity · 2026Article
- The structure of Shigella virus Sf14 reveals the presence of two decoration proteins and two long tail fibers.Communications biology · 2025Article
- Bacterial lysis or survival after infection with phage Sf14 depends on combined nutrient and temperature conditions.PloS one · 2025Article
- Environmental alkalization suppresses deployment of virulence strategies inJournal of bacteriology · 2024Article
- A Tale aboutMicroorganisms · 2023Review
- Translation factor accelerating peptide bond formation on the ribosome: EF-P and eIF5A as entropic catalysts and a potential drug targets.BBA advances · 2023Article
- Host Range Expansion ofJournal of virology · 2022Article
- Shigella flexneri Diguanylate Cyclases Regulate Virulence.Journal of bacteriology · 2021Article
- Large metabolic rewiring from small genomic changes between strains ofJournal of bacteriology · 2021Article
- The T3SS ofMicroorganisms · 2020Review
- Posttranslational modification of Elongation Factor P from Staphylococcus aureus.FEBS open bio · 2020Article
- Extragenic Suppression of Elongation Factor P Gene Mutant Phenotypes in Erwinia amylovora.Journal of bacteriology · 2019Article
- Density-dependent resistance protectseLife · 2019Article
- Human Intestinal Enteroids as a Model System ofInfection and immunity · 2019Article
- An Erwinia amylovora yjeK mutant exhibits reduced virulence, increased chemical sensitivity and numerous environmentally dependent proteomic alterations.Molecular plant pathology · 2018Article
- Evolutionary analysis of polyproline motifs in Escherichia coli reveals their regulatory role in translation.PLoS computational biology · 2018Article
- Regulatory Hierarchies Controlling Virulence Gene Expression inFrontiers in microbiology · 2018Review
- Miscoding-induced stalling of substrate translocation on the bacterial ribosome.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Article
- Neisseria meningitidis Translation Elongation Factor P and Its Active-Site Arginine Residue Are Essential for Cell Viability.PloS one · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Elongation factor P (EF-P) is a universally conserved bacterial translation factor. In many bacteria, EF-P is posttranslationally modified by PoxA, which covalently attaches a β-lysine to a conserved lysine residue of EF-P. Here we show that both EF-P and PoxA are necessary for virulence of the human diarrheal pathogen Shigella flexneri. Loss of either EF-P or PoxA leads to an impaired ability of S. flexneri to invade epithelial cells and form plaques in an epithelial cell monolayer. Proteomic analysis of efp and poxA deletion mutants revealed decreased levels of several virulence effector proteins, including IpaA, -B, and -C and IcsA. Additionally, mRNA levels of virB and virF, which encode master virulence regulators, were decreased in the efp mutant. The reduction in virF transcription was at least partially due to decreased levels of CpxA, which activates virF through the response regulator CpxR. The role of CpxAR in reduced synthesis of VirF and its downstream effectors was indicated by restoration of invasion when a mutation resulting in constitutively activated CpxR was introduced into the efp mutant. Thus, modified EF-P is required for appropriate synthesis of proteins involved in the virulence of this bacterial pathogen.
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