ArticleTissue engineering. Part A2014
Endothelial cell response to chemical, biological, and physical cues in bioactive hydrogels.
Article in Tissue engineering. Part A, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Mechanical maturation of human dermal fibroblast-laden microporous annealed particle scaffolds during long-term in vitro culture.Acta biomaterialia · 2025Article
- PEG-Based Hydrogel Coatings: Design Tools for Biomedical Applications.Annals of biomedical engineering · 2024Article
- Interpenetrating network design of bioactive hydrogel coatings with enhanced damage resistance.Journal of materials chemistry. B · 2023Article
- Prokaryotic Collagen-Like Proteins as Novel Biomaterials.Frontiers in bioengineering and biotechnology · 2022Review
- Review of Integrin-Targeting Biomaterials in Tissue Engineering.Advanced healthcare materials · 2020Review
- Bioactive hydrogel coatings of complex substrates using diffusion-mediated redox initiation.Journal of materials chemistry. B · 2020Article
- Hydrogel Small-Diameter Vascular Graft Reinforced with a Braided Fiber Strut with Improved Mechanical Properties.Polymers · 2019Article
- The Functional Implications of Endothelial Gap Junctions and Cellular Mechanics in Vascular Angiogenesis.Cancers · 2019Review
- A Review of Integrin-Mediated Endothelial Cell Phenotype in the Design of Cardiovascular Devices.Annals of biomedical engineering · 2019Review
- Introduction of sacrificial bonds to hydrogels to increase defect tolerance during suturing of multilayer vascular grafts.Acta biomaterialia · 2018Article
- High-throughput approaches for screening and analysis of cell behaviors.Biomaterials · 2018Review
- Engineering Biodegradable and Biocompatible Bio-ionic Liquid Conjugated Hydrogels with Tunable Conductivity and Mechanical Properties.Scientific reports · 2017Article
- Evaluation of late outgrowth endothelial progenitor cell and umbilical vein endothelial cell responses to thromboresistant collagen-mimetic hydrogels.Journal of biomedical materials research. Part A · 2017Article
- Substrate Stiffness Combined with Hepatocyte Growth Factor Modulates Endothelial Cell Behavior.Biomacromolecules · 2016Article
- Recombinant Collagen Engineered to Bind to Discoidin Domain Receptor Functions as a Receptor Inhibitor.The Journal of biological chemistry · 2016Article
- Collagen-mimetic hydrogels promote human endothelial cell adhesion, migration and phenotypic maturation.Journal of materials chemistry. B · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
The highly tunable biological, chemical, and physical properties of bioactive hydrogels enable their use in an array of tissue engineering and drug delivery applications. Systematic modulation of these properties can be used to elucidate key cell-material interactions to improve therapeutic effects. For example, the rate and extent of endothelialization are critical to the long-term success of many blood-contacting devices. To this end, we have developed a bioactive hydrogel that could be used as coating on cardiovascular devices to enhance endothelial cell (EC) adhesion and migration. The current work investigates the relative impact of hydrogel variables on key endothelialization processes. The bioactive hydrogel is based on poly(ethylene glycol) (PEG) and a streptococcal collagen-like (Scl2-2) protein that has been modified with integrin α1β1 and α2β1 binding sites. The use of PEG hydrogels allows for incorporation of specific bioactive cues and independent manipulation of scaffold properties. The selective integrin binding of Scl2-2 was compared to more traditional collagen-modified PEG hydrogels to determine the effect of integrin binding on cell behavior. Protein functionalization density, protein concentration, and substrate modulus were independently tuned with both Scl2-2 and collagen to determine the effect of each variable on EC adhesion, spreading, and migration. The findings here demonstrate that increasing substrate modulus, decreasing functionalization density, and increasing protein concentration can be utilized to increase EC adhesion and migration. Additionally, PEG-Scl2-2 hydrogels had higher migration speeds and proliferation over 1 week compared with PEG-collagen gels, demonstrating that selective integrin binding can be used to enhance cell-material interactions. Overall, these studies contribute to the understanding of the effects of matrix cues on EC interactions and demonstrate the strong potential of PEG-Scl2-2 hydrogels to promote endothelialization of blood-contacting devices.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.