ArticleBMC veterinary research2014
Detection of serum antibodies to hepatitis E virus in domestic pigs in Italy using a recombinant swine HEV capsid protein.
Article in BMC veterinary research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- Changes in Hepatitis E Virus Contamination during the Production of Liver Sausage from Naturally Contaminated Pig Liver and the Potential of Individual Production Parameters to Reduce Hepatitis E Virus Contamination in the Processing Chain.Pathogens (Basel, Switzerland) · 2024Article
- Cross-sectional study of hepatitis E virus (HEV) circulation in Italian pig farms.Frontiers in veterinary science · 2023Article
- Quantitative Methods for the Prioritization of Foods Implicated in the Transmission of Hepatititis E to Humans in Italy.Foods (Basel, Switzerland) · 2021Article
- Article
- Increasing Hepatitis E Virus Seroprevalence in Domestic Pigs and Wild Boar in Bulgaria.Animals : an open access journal from MDPI · 2020Article
- Seroprevalence of Hepatitis E Virus in Forestry Workers from Trentino-Alto Adige Region (Northern Italy).Pathogens (Basel, Switzerland) · 2020Article
- Pilot Investigation on the Presence of Anti-Hepatitis E Virus (HEV) Antibodies in Piglet Processing Fluids.Animals : an open access journal from MDPI · 2020Article
- Liver Transudate, a Potential Alternative to Detect Anti-Hepatitis E Virus Antibodies in Pigs and Wild Boars (Microorganisms · 2020Article
- Nine-Year Nationwide Environmental Surveillance of Hepatitis E Virus in Urban Wastewaters in Italy (2011-2019).International journal of environmental research and public health · 2020Article
- Hepatitis E in pigs in Israel: seroprevalence, molecular characterisation and potential impact on humans.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2018Article
- Hepatitis E virus infection in North Italy: high seroprevalence in swine herds and increased risk for swine workers.Epidemiology and infection · 2017Article
- Public health risks associated with hepatitis E virus (HEV) as a food-borne pathogen.EFSA journal. European Food Safety Authority · 2017Article
- Xenotransplantation - A special case of One Health.One health (Amsterdam, Netherlands) · 2017Review
- Hepatitis E Virus (Genotype 3) in Slurry Samples from Swine Farming Activities in Italy.Food and environmental virology · 2017Article
- Hepatitis E in Norway: seroprevalence in humans and swine.Epidemiology and infection · 2017Article
- A Novel Blocking ELISA for Detection of Antibodies against Hepatitis E Virus in Domestic Pigs.PloS one · 2016Article
- Hepatitis E virus genotype 3 diversity: phylogenetic analysis and presence of subtype 3b in wild boar in Europe.Viruses · 2015Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe hepatitis E virus (HEV) has been detected in both humans and animals, particularly pigs, worldwide. Several evidences, including human infection following consumption of raw contaminated meat, suggest a zoonotic transmission of HEV. In Italy, large circulation of genotype 3 HEV has been reported in swine, and recent studies have confirmed the involvement of this genotype in autochthonous human cases.
resultIn this study 111 sera collected from healthy pigs in two Italian regions were tested for anti-HEV IgG antibodies. For specific HEV antibody detection in swine, we developed ELISA and Western blotting methods, using a truncated capsid (ORF2) protein lacking the first 111 amino acids of a swine HEV genotype 3 strain. The ORF2-based ELISA revealed anti-HEV antibodies in 104 out of 111 pigs compared with 102 detected with a commercial ELISA kit. A lower number of sera reacted with the recombinant ORF2 protein in a Western blotting format (81/111). Using a Latent class analysis (LCA), the estimated sensitivities for ELISA-ORF2 and ELISA-kit tests were 0.961 and 0.936, respectively, whereas specificities were 0.599 and 0.475. The estimated sensitivity of Western blotting was 0.775, and the specificity was 0.944.
conclusionsThe overall results confirm the high prevalence of HEV seropositive healthy pigs in Italy. Through comparisons with a commercial ELISA test, the swine genotype 3 HEV antigen produced in this study was proven suitable to detect anti-HEV antibodies in pig sera by both ELISA and Western Blotting.
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