ArticleInternational journal of molecular sciences2014
An efficient Agrobacterium-mediated transformation system for poplar.
Article in International journal of molecular sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Genetically Modified Plants in Agriculture.Biology · 2026Review
- Organic extracts from sustainable hybrid poplar hairy root cultures as potential natural antimicrobial and antibiofilm agents.Protoplasma · 2024Article
- Simplified Method forMethods and protocols · 2024Article
- Optimization of High-Efficiency Tissue Culture Regeneration Systems in Gray Poplar.Life (Basel, Switzerland) · 2023Article
- CRISPR-mediated genome editing in poplar issued by efficient transformation.Frontiers in plant science · 2023Article
- Genome-wide identification, expression analysis, and potential roles under low-temperature stress of bHLH gene family inFrontiers in plant science · 2023Article
- An EfficientInternational journal of molecular sciences · 2022Article
- Precise exogenous insertion and sequence replacements in poplar by simultaneous HDR overexpression and NHEJ suppression using CRISPR-Cas9.Horticulture research · 2022Article
- Isoprenoid biosynthesis regulation in poplars by methylerythritol phosphate and mevalonic acid pathways.Frontiers in plant science · 2022Article
- Optimization of parameters to improve transformation efficiency of elephant foot yam (3 Biotech · 2021Article
- EfficientInternational journal of molecular sciences · 2019Article
- Simple, rapid and efficient transformation of genotype Nisqually-1: a basic tool for the first sequenced model tree.Scientific reports · 2017Article
- Agrobacterium-Mediated Stable Genetic Transformation of Populus angustifolia and Populus balsamifera.Frontiers in plant science · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Poplar is a model system for the regeneration and genetic transformation of woody plants. To shorten the time required for studies of transgenic poplar, efforts have been made to optimize transformation methods that use Agrobacterium tumefaciens. In this study, an Agrobacterium infective suspension was treated at 4 °C for at least 10 h before infecting explants. By transforming the Populus hybrid clone "Nanlin895" (Populus deltoides×P. euramericana) with Agrobacterium harboring the PBI121:CarNAC6 binary vector, we showed that the transformation efficiency was improved significantly by multiple independent factors, including an Agrobacterium infective suspension with an OD600 of 0.7, an Agrobacterium infection for 120 min, an Agrobacterium infective suspension at a pH of 5.0, an acetosyringone concentration of 200 µM, a cocultivation at 28 °C, a cocultivation for 72 h and a sucrose concentration of 30 g/L in the cocultivation medium. We also showed that preculture of wounded leaf explants for two days increased the regeneration rate. The integration of the desired gene into transgenic poplars was detected using selective medium containing kanamycin, followed by southern blot analysis. The expression of the transgene in the transgenic lines was confirmed by northern blot analysis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.